Jul 6, 2008 · We show here that superoxide dismutase 1 (SOD1) regulates caspase-1 activation. In SOD1-deficient macrophages, higher superoxide production decreased the cellular redox potential and...

Stable overexpression of SOD1 caused significant decreases in superoxide and nitric oxide production, with concurrent increases in hydrogen peroxide following LPS. However, LPS-induced activation of NFkappaB, and release of TNFalpha and IL-6 were significantly attenuated in SOD1 overexpressing cells, as was the ability of microglia to induce ...

Adenovirus-mediated gene transfer of SOD1 decreases vascular O 2 •− levels in atherosclerosis and diabetes (158, 249) to improve endothelial function in diabetes , and protects endothelial function in vascular tissues with aging , treated with various oxidative stimulants such …

Superoxide dismutase 1 (SOD1) is an enzyme that is important in defending tissues against oxidative stress; it acts by catalyzing the conversion of superoxide radicals (O 2−) to hydrogen peroxide, which can then be further reduced to water [1].

Apr 9, 2019 · We used the N9 microglia cell line to identify phenotype diversity upon the overexpression of wild-type (WT; hSOD1 WT) and mutated G93A (hSOD1 G93A) protein. To investigate how each transduced cell respond to an inflammatory stimulus, N9 microglia were treated with lipopolysaccharide (LPS).

We exposed wild-type and end-stage SOD1 G93A rats to LPS (i.p. 5mg/kg) for 20 hours, and analyzed microglial responses. In wild-type rats, microglia in all spinal regions displayed stronger pro-inflammatory responses to LPS than microglia from cortex.

Mar 29, 2017 · Murine microglial hSOD1 (G93A) cells express all the inflammasome components and LPS activates caspase1 leading to an increase in the secretion of IL-1β. By activating NF-κB, LPS increases ROS and NO levels that spontaneously react to form peroxynitrite, thus leading to protein nitration.

Mar 5, 2025 · Treatment with CGS21680 significantly restored expression levels of OS-related genes (SOD1, SOD2, Gpx1 and GSS) and increased HO-1 expression in LPS-induced mice (Figure 4B). CGS21680 treatment also reduced kidney …

Jul 27, 2020 · Here we report that knocking out these three factors markedly extends survival in the SOD1G93A ALS mouse model, providing evidence for gliosis as a potential ALS therapeutic target.

Jan 1, 2011 · In the present study, we used SOD1 knockout (SOD1-/-), GPX1 knockout (GPX1-/-), SOD1 and GPX1 double-knockout (DKO), and their wild-type (WT) mice to investigate the impact of knockout of these two enzymes on the LPS-induced hepatic oxidative injury in mice.