IL-1β is transcriptionally and post-translationally regulated and is primarily induced after recognition of microbial patterns (5, 6). Monocytes appear to be the main cell that produces IL-1β following stimulation with LPS.

Jan 4, 2021 · To aid in a thorough mechanistic inquiry of CASP8-mediated cell death, we used bone marrow-derived macrophages (BMDMs) activated with lipopolysaccharide (LPS) and the small-molecule inhibitor of...

In this study, we investigate the effect of MyD88 dimerisation inhibitor ST2825 on cytokine production from rhIL-1β and LPS-stimulated peripheral blood mononuclear cells (PBMC) from healthy blood donors (HBD).

Interleukin-1β (IL-1β) is a potent pro-inflammatory cytokine that is crucial for host-defence responses to infection and injury [1]. It is also the best characterised and most studied of the 11 IL-1 family members.

Feb 12, 2016 · To stimulate the release of IL-1β, cells were primed with bacterial endotoxin (LPS, 1 μg/ml for macrophages or 100 ng/ml for neutrophils, 4 h – signal 1) and then treated with ATP (5 mM, from...

In this study, we used lipopolysaccharide (LPS) and adenosine triphosphate (ATP) to simulate an inflammatory environment as the testing model. We found that the exposure of RAW264.7 cells to LPS/ATP triggered the activation of caspase-1 (P<0.01) and the cleavage of interleukin (IL)-1β (P<0.01), as well as the release of other cytokines, such ...

Upon lipopolysaccharide (LPS) triggering of toll-like receptor (TLR)-4 and subsequent ATP signaling, the NOD-like receptor containing-pyrin domain 3 (NLRP3) inflammasome is activated to cleave pro-caspase-1 into caspase-1, allowing the secretion of IL-1β.

Mar 1, 2020 · IL1A, IL1B, and IL1RN were rapidly induced after stimulation with LPS; however, IL1B mRNA production was less inhibitable by iBET151, suggesting it does not use pause-release regulation.

Jul 15, 2021 · LPS induced the expression of the inflammasome components NLRP3, NLRC4 and ASC, the secretion of the cytokines IL-1b and IL-18 and the activation of caspase-1 protease in BV2 cells. Pre-treatment with CXCL12 significantly reduced LPS-induced IL-1b/IL-18 secretion and inflammasome induction.

Jul 1, 2023 · Here, we investigated the role of the NLRP3 inflammasome in a murine model of lipopolysaccharide (LPS)-induced SCM. LPS injection induced cardiac dysfunction, damage, and lethality, which was significantly prevented in NLRP3−/− mice, compared to wild-type (WT) mice.