Sep 23, 2022 · Here, we report a method based on the multi-laser cylindrical illumination confocal spectroscopy (CICS) technique to examine mRNA and lipid contents in LNP formulations at …

Oct 6, 2021 · We found that a low (high) molar ratio of PEG improves the performance of LNPs with neutral (cationic) helper lipids, an identified and optimal LNP for low-dose messenger …

Jul 12, 2019 · Lipid nanoparticles (LNPs) have been widely used for RNA formulations, where the prevailing paradigm is to encapsulate RNA within the particle, including the first FDA-approved...

Mar 1, 2023 · As visualized by cryo-TEM, LNP exhibited a conventional solid core that decreased in size as the DSPC-cholesterol level was increased from 5 to 60 mol%; and then showed a …

Mar 18, 2025 · The morphology of LNP-mRNA was analyzed by cryogenic transmission electron microscopy (cryo-TEM) using the plunge freezing technique whereby the samples were …

Aug 1, 2023 · Cryogenic electron microscopy, including cryogenic transmission EM (cryo-TEM), is a technique used to image frozen LNP samples, revealing their size, size distribution and …

Apr 5, 2024 · Lipid nanoparticles (LNPs) have emerged as promising carriers for delivering nucleic acid-based therapeutics and vaccines. Comprehensive characterization of LNPs is …

Contrary to liposomes which have a double membrane and are observable by in-situ TEM [8], LNP could not be observed without functionalizing their surface. Particle grafting with metallic …

Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. LNPs are relatively immunologically inert and can be produced in a cost effective …

Patisiran is a lipid nanoparticle (LNP)-formulated siRNA used for the treatment of hereditary transthyretin-mediated amyloidosis. In contrast, givosiran is based on siRNA conjugated with a …