LPS stimulation of CD14-expressing cells induced TLR4 internalization in the presence of serum, and an inhibitory anti-LBP mAb blocked its internalization. Addition of LBP to serum-free …

In this review, we focus on the mechanisms of the internalization and intracellular trafficking of TLR4 and CD14, and also of LPS, in immune cells and discuss how dysregulation of the endo …

Jun 29, 2018 · Here, we investigated the mechanisms of LPS-induced TLR4 internalization and clarified the roles of the extracellular LPS-binding molecules, LPS-binding protein (LBP), and …

CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. [5] [6] It helps to detect bacteria in the body by binding …

Dec 6, 2013 · LBP then transfers LPS to CD14, which can be found either in soluble form or linked to the cell surface by a glycosylphosphatidylinositol anchor. CD14 splits LPS …

Mar 18, 2025 · CD14 plays a pivotal role in the LPS-TLR4 signaling pathway by recognizing LBP-delivered LPS and presenting it to TLR4, making LPS the first and most extensively studied …

In this study, we examined the role of LBP and CD14 in the physical generation of the ternary TLR1·TLR2·lipopeptide complex by performing biophysical measurements with functional …

LPS binding protein (LBP) and CD14 play key roles in promoting innate immunity to Gram-negative bacteria by transferring LPS to the signaling receptor complex, MD-2/Toll-like …

Dec 1, 2023 · Lipoteichoic acid (LTA) of Streptococcus pneumoniae and Staphylococcus aureus activates immune cells via Toll-like receptor (TLR)-2, lipopolysaccharide-binding protein (LBP), …

LPS transfer to TLR4-MD2 is catalyzed by both LPS binding protein (LBP) and CD14. To define the sequential molecular interactions underlying this transfer, we reconstituted in vitro the …