We investigated the role of changes in sHSP oligomerization by studying a mutant with reduced oligomeric stability. In HSP16.6, the single sHSP in the cyanobacteriumSynechocystis sp. PCC 6803, the mutation L66A causes oligomer instability and reduced chaperone activity in vitro.

Jul 30, 2004 · We identified a mutation of Synechocystis Hsp16.6 that impairs function in vivo and in vitro. The V143A mutation is in the C-terminal extension, a region predicted to form an oligomeric interaction with a hydrophobic region that includes the site of a previously characterized mutation, L66A.

May 1, 2012 · However, a cytoplasmic distribution of the EGFP-NES mutants was observed for only 44.0% ± 18.0% to 64.7% ± 4.5% of the transfected cells, indicating that the I59A, V63A, L66A, or V68A mutations of the Flu-A-M1 NES impair the function of the NES.

Strains containing mutated TtgR, which showed higher affinity for the ttgR-ttgA promoter region (L66A and L66A/V96A), showed increased susceptibility to antibiotics when compared with native TtgR containing cells.

Jan 20, 2007 · On the basis of these studies, we propose DNA-binding models for the interaction between the RFX complex and the MHCII promoter including a DNA looping model. We also provide direct evidence that the RFX5 (L66A) point mutation prevents dimerization of the RFX complexes and propose a model for how this results in a loss of MHCII gene expression.

Feb 2, 2010 · In this study, we examined the role of the ten amino acids within the predicted second α-helical domain of the subtype B Vpu cytoplasmic tail in CD4 down-modulation using a VpuEGFP reporter system.

Feb 13, 2007 · We also provide direct evidence that the RFX5 (L66A) point mutation prevents dimerization of the RFX complexes and propose a model for how this results in a loss of MHCII gene expression. Major histocompatability complex class II (MHCII) molecules are an essential component of the mammalian adaptive immune response.

Download scientific diagram | Substitutions of L66A and V68A in M1 damage virus replication. 293T cells were cotransfected with the 12 plasmids for virus rescue, as mentioned in Materials and...

Reactivity of CTL clone, directed against the M1 58-66 epitope, with stimulator cells infected with wild type (WT) (B) or influenza virus A/NL/95-M1 G58A (C), L60A (D), G61A (E), F62A (F), V63A (G), T65A (H), or L66A (I) was determined by intracellular IFN-γ staining and flow cytometry. PE, phycoerythrin conjugate; FITC, fluorescein ...

All 11 HRD residues, including H2A-L66, are highly conserved in extant eukaryotic cells; therefore, our screening reported in this study will provide a foundation for future studies about the mechanisms underlying eukaryotic HR based on chromatin.