L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The extent of cell fusion declines with passage and the cells should be frozen at low passage and periodically recloned with selection for fusion competent cells.

Immortalized rat skeletal muscle cells (L6) over-expresses GLUT4 with a myc-epitope, which is useful for measuring GLUT4 translocation. Highlights: L6 myoblast cell line that stably expresses GLUT4 protein containing a fourteen amino acid epitope of …

When stimulated by insulin, L6 myotubes appeared to exhibit glucose uptake to a larger extent than C2C12 cells (Sarabia et al. 1990), suggesting that at present L6 myotubes could be the most promising candidate as an efficient in vitro model system to investigate glucose uptake in …

GLUT-4myc ectopic expression in L6 myoblasts generates a GLUT-4-specific pool conferring insulin sensitivity. Farewell to professor David Yaffe -- a pillar of the myogenesis field.

Differentiated L6 myoblast cell line is the best characterized cellular model of skeletal muscle origin to study glucose uptake and GLUT4 translocation. L6 myoblasts were maintained in DMEM supplemented with 10% FBS, 10% antibiotic-antimycotic mix at 37°C under 5% CO 2 …

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health.

Immortalized rat skeletal (L6) myoblast cell line that was selected for high fusion potential and endogenous expression of GLUT4 in the myotube stage. Highlights: Differentiate with high reliability into a myotube muscle cell phenotype that naturally expresses the GLUT4 glucose transporter protein

L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The extent of cell fusion declines with passage and the cells should be frozen at low passage and periodically …

A rat skeletal muscle cell line (L6) has been used as a model to assess the muscle irritancy potential of several parenteral antibiotics.

Nov 1, 2023 · This paper highlights four cell models for skeletal muscle atrophy: C2C12, L6, primary cells and iPSCs. We discuss factors including hormones, inflammatory factors, cachexia, drugs, genes, and physical-chemical factors.