Making sense of enzyme Km comparisons - Biology Stack Exchange
The Km value of glucokinase is 10(micromolar), whereas the (Km) value for hexokinase-1 is 0,1(micromolar). Therefore glucose affinity of glucokinase is low.". Now, lets say glucokinase …
What is the meaning behind Kcat / Km? - Biology Stack Exchange
Nov 19, 2016 · You'd just have the initial slope of the [S] vs vmax graph. At low concentrations this slope approaches kcat/Km. The kcat/Km ratio doesn't represent something real, it's just a …
{K}_{m} value isMaximum reaction velocityOne half of maximum …
Km (Michaelis Menten) indicates that substrate concentration attains half its maximum velocity when enzymes catalyze the chemical reaction. Km values generally lies between 10-1 to 10 …
K m value of enzyme is substrate concentration at - Toppr
The catalytic efficiency of an enzyme is described by the K m value or Michaelis Menten constant. The Michaelis constant is the substrate concentration at which the reaction rate is one half the …
K m value of enzyme is substrate concentration at - Toppr
A smaller k m value indicates greater affinity of the enzyme for its substrate, hence, shows a quicker reaction k m is a constant characteristic of an enzyme for its conversion of a substrate. …
What is exhibited by lower Km value?More affinity with ... - Toppr
A smaller k m value indicates greater affinity of the enzyme for its substrate, hence, shows a quicker reaction k m is a constant characteristic of an enzyme for its conversion of a substrate. …
km value and Vmax - Protein and Proteomics - Protocol Online
Jul 18, 2006 · Km is the concentration of substrate required to produce 50% of the Vmax value. It is measured in units of concentration (generally M). Competitive and non-competitive inhibitors …
What are the structural factors affect enzyme's Km?
What causes binding? Well, any interaction between the enzyme and the substrate, including H-bonds, salt bridges, hydrophobic pocket on the enzyme that can accommodate the …
What happens to Km when enzyme concentration is *very* high?
Let Km be an empirical measurement of a certain enzyme with concentration [E]. Theoretically, this value is constant and shouldn't vary when [E] goes up or down. Now let [E']=10*Km. …
Km Vmax - Biochemistry
The Km value is important because the concentrations you use in assays need to cover a range surrounding the Km value. Question 4: in vitro vs in vivo Km values. In theory, if the conditions …