Kcat is turnover number, expresed as number of substrate molecules turned into product per enzyme site per minute. Km and X are both in the same units of substrate concentration. …

All you have to do is find the [Et]. This is basically the total "Moles" of the enzymes you have used. which comes out to be 10^-10. Then you find the kcat = Vm/[Et], then find kcat/km

Aug 12, 2020 · In other words, a high kcat/Km ratio means the enzyme works well with not much substrate. This is called catalytic efficiency because if the enzyme is efficient, it means it …

Km is a substrate concentration and is the amount of substrate it takes for an enzyme to reach V max /2. On the other hand V max /2 is a velocity and is nothing more than that. The value of …

Kcat is the turnover number -- the number of substrate molecule each enzyme site converts to product per unit time. If you know the concentration of enzyme sites, you can fit Kcat instead …

As a beginner, you may find it easier to use Eisenthal & Cornish-Bowden's "direct linear plot" of your data to determine Vmax and Km than a regression of v against [S]. As a non-parametric...

Jun 13, 2022 · Although the parameter provides critical information on how the enzyme lowers the energy barriers along the reaction pathway for catalysis, kcat / KM is frequently considered as …

The kcat/km ratio is a measure of the catalytic efficiency of an enzyme. It tells us how fast an enzyme can convert a substrate into a product under different conditions. In this post, we will …

Kcat and Km are both important parameters used to characterize enzyme kinetics. Kcat, also known as the turnover number, represents the maximum rate at which an enzyme can …

Nov 19, 2016 · Km , the substrate concentration at which the reaction rate is half of V max. Kcat, used to describe the limiting rate of any enzyme-catalyzed reaction at saturation. Most of the …