Ki67 is crucial for maintaining chromosome dispersion in the cytoplasm during cell division. Widely used in research and pathology as a tumor marker. Valuable for prognosis and diagnosis of various cancers. Ki67 index correlates with disease progression and can assess patient survival and tumor progression.

We examined data from 374 laboratories staining for Ki-67 as part of external quality assessment over 8 runs between 2013 and 2017 (total data sets=2601). One of 5 primary antibodies was used for 94.8% of submissions, with MIB-1 (Agilent Dako) comprising 58.8% of the total.

Dual ICC staining with Ki-67 and CD3 antibodies was performed to assess the percentage of Ki-67-positive lymphoma B cells (Ki-67 PI) and T cells. The ICC staining results were assessed by two different methods: (1) manual counting and (2) eyeballing.

May 18, 2016 · Pathological analysis of the nuclear proliferation biomarker Ki67 has multiple potential roles in breast and other cancers. However, clinical utility of the immunohistochemical (IHC) assay for...

Ki67 prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PMID: 27362226).

Dec 24, 2015 · Ten of the 20 breast cancers had hot spots of Ki-67 expression. Each observer scored Ki-67 in two different ways: direct counting (average vs. hot spot method) and categorical estimation. Intraclass correlation coefficient (ICC) of Ki …

Apr 3, 2025 · The mean Ki-67 scores from senior pathologists and the algorithm were 23 ± 18 and 24 ± 18, respectively (ICC, 0.98). Ki-67 scores from the residents were 19 ± 16 and 22 ± 16, without and with input from the algorithm, respectively. With input from the algorithm, residents’ scores were significantly closer to those obtained by senior ...

ICC tips. Ki67 is located in the nucleus, so you need to permeabilize cells for successful immunostaining. We recommend following the tips below for IHC.

Purpose: Ki-67 has been clinically validated for risk assessment in breast cancer, but the analytical validation and cutpoint-definition remain a challenge. Intraclass correlation coefficients (ICCs) are a statistical parameter for Ki-67 interobserver performance.

4 days ago · The interclass correlation coefficient (ICC) for each feature was calculated to assess reliability, and features with an ICC greater than 0.75 were retained, narrowing the selection down to 415 features. ... Sun X, Kaufman PD. Ki-67: more than a proliferation marker. Chromosoma. 2018;127(2):175–86. Article CAS PubMed PubMed Central Google ...