Dec 15, 2021 · KpnI has a High Fidelity version KpnI-HF ® (NEB #R3142). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means …

Thermo Scientific FastDigest KpnI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Acc65I is an isoschizomer of KpnI. KpnI produces a 4 …

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In …

Kpn I is an isoschizomer of Asp 718 which generates fragments with 5' cohesive termini. Kpn I is not inhibited by 5-methylcytosine at either or both C-residues or by 6-methyladenine. 10 mM 2 …

Kpn I is a DNA restriction enzyme that is used in molecular biology research to cleave DNA at the recognition sequence 5′-GGTAC/C-3′ to generate DNA fragments with 3′-cohesive termini. …

KpnI is a restriction endonuclease that is used in molecular biology applications to cleave the DNA recognition site 5′-GGTAC/C-3′, resulting in DNA fragments with 3′-cohesive termini. This type …

Kpn I is an isoschizomer of Asp 718 I (which generates fragments with 5′-cohesive termini). Kpn I is not inhibited by 5-methylcytosine at either or both C-residues in the recognition sequence, …

起源: Escherichia coli carrying the plasmid encoding Kpn I gene: 活性测定用底物: λ DNA: 甲基化的影响: 不受CG methylase的影响。 使用上的注意: 本酶在过量情况下长时间反应容易出 …

The restriction endonuclease (REase) R.KpnI is an orthodox Type IIP enzyme, which binds to DNA in the absence of metal ions and cleaves the DNA sequence 5′-GGTAC^C-3′ in the …