Adoptive transfer of CD19-specific chimeric antigen receptor (CD19CAR) T cells can induce dramatic disease regression in patients with B cell malignancies. CD19CAR T cell therapy may be limited by insufficient engraftment and persistence, resulting in tumor relapse.

Cytotoxicity of CD19-CAR T cells against BV173 (CD19 +, German Collection of Microorganisms and Cell Cultures) or KG1a (CD19 −, American Type Tissue Collection) target cells was determined in a flow cytometry-based cytotoxicity assay.

Nov 1, 1986 · Both KG1 and KG1a transcribe unrearranged IgH genes. These data suggest that either the KG1 cell line was derived from a common myeloid-lymphoid progenitor or that the KG1a subline phenotype is aberrant.

CD19-ENG T cells killed CD19 + (BV173) but not CD19 − (KG1a) tumor cells, and expression of CD80 and/or 41BBL did not change their specificity or cytolytic activity (Fig. 3A).

Oct 31, 2013 · To examine the in vivo antileukemic activity of CD123 CAR T cells, we developed a xenogeneic model of systemic AML using the KG1a leukemic cell line engineered to express the eGFP and ffLuc reporter genes (KG1a-eGFP-ffLuc).

Dec 2, 2016 · Conclusions: We have generated for the first time NK cells that kill B-cell malignancies through a CAR (CD22) and simultaneously redirect bystander T cells to a 2 nd B-cell antigen (CD19) to enhance antitumor effects and prevent immune escape.

Autologous chimeric antigen receptor (CAR) T cells targeting the CD19 antigen have demonstrated a high complete response rate in relapsed/refractory B-cell malignancies.

ENG T cells were plated in the insert well and KG1a target cells with NT T cells in the bottom well. Only CD123-ENG T cells, in contrast to NT T cells and CD19-ENG T cells, were able to induce significant target killing by NT T cells (Figure 3a).

Nov 1, 1986 · In marked contrast, its subline KG1a has lost myeloid features, acquired new karyotypic markers, and has three characteristics associated with immature T cells: low-level expression of the T cell...

Mar 20, 2015 · HL-60 cells are a model for human myeloid promyelocytic cell differentiation and proliferation, and KG1a is a model for immature myeloid progenitor phenotype and FGFR1 kinase fusion genes (Table...