Learn about JM109 competent cells, a classic E. coli strain for routine cloning and plasmid maintenance. E. coli K12 JM109 is recombination- and endonuclease-deficient, improving insert stability and quality of miniprep DNA.

JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening.

Bacterial Strain JM109 is a useful host for transformation of pGEM® Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods.

Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site.

As TaKaRa E.coli JM109 Competent Cells contains F' plasmid, it can be used as a host of M13 vector DNA as well as for preparation of DNA library or subclon-ing. When transformation of pUC vectors or transduction of M13 phage vector DNAs, recombinants can be selected easily by adding X-Gal and IPTG to a me-

JM109 cells are endonuclease (endA) deficient, greatly improving the quality of miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the Eco K endonuclease system.

E. coli JM109は、pUC系プラスミドベクターDNAによる形質転換やM13ファージベクターDNAによる形質導入等を行う際に、ベクターDNAより生成するlacZαペプチドとJM109F'にコードされるlacZΔM15とによるβ-ガラクトシダーゼの活性回復（α-相補性）を利用することにより ...

GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning.

JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heterologous DNA by an endogenous endonuclease.

JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F' episome, which allows blue/white screening.