
JC-1 Dye for Mitochondrial Membrane Potential | Thermo …
Track mitochondrial membrane potential with JC-1 dye. Flow Cytometry & Imaging kits. Detect membrane potential in a variety of cell types and in intact tissues.
Cells labeled with JC-1 can be analyzed by flow cytometry using 488 nm excita-tion and green or orange-red emission, and by fluorescence microscopy using standard filters for Alexa Fluor ® …
Analysis of the Mitochondrial Membrane Potential Using the …
In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green fluorescence) which is able to enter into the mitochondria where it accumulates and (in a concentration-dependent …
JC-1: alternative excitation wavelengths facilitate ... - Nature
Nov 22, 2012 · JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this approach is not optimal for …
MitoProbe™ JC-1 Assay Kit - Thermo Fisher Scientific
The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry. View a selection guide for all apoptosis assays for flow …
Flow cytometric analysis of mitochondrial membrane potential …
This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial …
In healthy cells, JC‐1 enters the energized mitochondria and form aggregates which change the fluorescent property of JC‐1 dye. JC‐1 monomers exhibit green fluorescence where as the …
; accessed 14th April 2015) The following protocol describes introducing JC-1 reagent into cultured cells and analyzing the stained cells by flow cytometry.
eBioscience™ JC-1 Mitochondrial Membrane Potential Dye
JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the …
Labeling Mitochondria with JC-1 - CSH Protocols
JC-1 has also been useful in flow-cytometry studies, because the membrane potential can be followed without the need for confocal microscopy. This protocol describes the labeling of …
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