Vector with a minimal promoter and an interferon-stimulated response element for studying cell signaling using destabilized luciferase. Explore Over 2.7k Plasmids: Luciferase Vectors | More …

The ISRE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.

As an example we analyzed the activity of two selected artificial promoters: The interferon response element (ISRE) stimulated by type I and III IFN (Stark et al., 1998) and the gamma-activated sequence (GAS) induced upon type II IFN signaling.

This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of an interferon-sensitive response element (ISRE) promoter. This cell line was established through lentiviral transduction and single cell cloning.

ISRE-Luc萤光素酶报告基因质粒主要应用于检测细胞IFN (Type I interferon)信号通路、Th1/Th1细胞分化等相关基因的调控和RNAi的表型分析等。 ISRE-Luc是翌圣生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个STAT1/STAT2结合位点，可以高灵敏度地检测STAT1/STAT2的激活水平。 同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，在保持原有功能不变的情况下，增加了质粒的转录因子结合特异性。 另外，由于质粒体积减小，使 …

ISRE-Luc Reporter Cell Line-HEK293 stably expresses luciferase reporter gene under the control of the Interferon-Stimulated Response Element (ISRE). This cell line is an ideal cellular model for monitoring JAK/STAT signaling pathway activities.

A protocol for use of pGL4.45, a luciferase reporter vector containing an interferon-stimulated response element (ISRE) that drives transcription of the luciferase gene luc2P.

THP1-Lucia™ ISG cells express the secreted luciferase (Lucia) reporter gene under the control of an IRF-inducible promoter. This composite promoter is comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-kB or AP-1 pathways.

Jun 1, 2023 · Neo-ISRE-Luc plasmid was provided by Dr. P.-O. Vidalain (VIRal Infection Metabolism Immunity team, CIRI, Lyon, France). This plasmid encodes for a firefly luciferase reporter gene under the control of a promoter containing five ISRE copies and has neomycin resistance gene.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. we constructed a luciferase reporter cell line that co-expresses luciferase and target protein by stimulation. When you …