Dec 1, 2021 · We examined ISRE binding patterns under IFN stimulation across six cell types using existing ChIPseq datasets. We find that ISRE binding is largely cell specific for ISREs distal to transcription start sites (TSS) and largely conserved across cell types for …

Dec 23, 2013 · Type I interferon (IFN) responses are regulated by host, pathogen and environmental factors. These factors calibrate the host defences while limiting tissue damage and preventing autoimmunity.

Uccellini and García-Sastre create an ISRE reporter mouse and track interferon (IFN) responses in vivo in response to pathogen-associated molecular pattern (PAMP) stimulation and influenza infection. They find that IFN responses are highest in hematopoietic cells during infection.

Interferon (IFN)-I and IFN-II both induce IFN-stimulated gene (ISG) expression through Janus kinase (JAK)-dependent phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT2.

Electrophoretic mobility-shift assay and site-directed mutagenesis revealed that interferon-sensitive responsive element (ISRE) and STAT1 and STAT2 are primarily responsible for the constitutive expression of PD-1, as well as for the IFN-alpha-mediated upregulation of PD-1.

The interferon (IFN) pathway plays a critical role in the human immune response. Following viral infection, the human body triggers a complex regulatory system of innate and adaptive immune responses designed to defend against the virus.

Here, we apply luciferase reporter gene assays to rapidly determine the impact of SARS-CoV-2 proteins on interferon (IFN) signaling pathways (for comprehensive reviews see (Platanias, 2005; Randall and Goodbourn, 2008; Stark et al., 1998; Stetson and Medzhitov, 2006)).

Sep 8, 2020 · We examined ISRE binding patterns under IFN stimulation across six cell types using existing ChIPseq datasets available on the GEO and ENCODE databases.

To elucidate the host cell defense mechanisms in response to Sindbis viral infection, we have started to characterize interferon (IFN)-stimulated response element (ISRE)-binding proteins activated in infected cells that are involved in the transcriptional induction of IFN type I …

Dec 4, 2018 · Uccellini and García-Sastre create an ISRE reporter mouse and track interferon (IFN) responses in vivo in response to pathogen-associated molecular pattern (PAMP) stimulation and influenza infection. They find that IFN responses are …