WHAT IS THE ISO-SEQ METHOD? The PacBio Iso-Seq method is an end-to-end workflow for sequencing and analyzing full-length transcript isoforms. WHY IS FULL-LENGTH RNA …
Introduction of the Iso-Seq method: state of the art for full-length ...
May 3, 2018 · Gene regulation through alternative splicing can dramatically increase the protein-coding potential of a genome. Understanding the functional biology of a genome requires …
Iso-Seq Home | Iso-Seq Docs
IsoSeq contains the newest tools to identify transcripts in PacBio single-molecule sequencing data. Starting in SMRT Link v6.0.0, those tools power the Iso-Seq GUI-based analysis …
RNA sequencing - PacBio
The Iso-Seq method sequences the entire cDNA molecules – up to 10 kb or more – without the need for bioinformatics transcript assembly, so you can characterize novel genes and isoforms …
Iso-Seq - GENEWIZ from Azenta Life Sciences
Isoform sequencing (Iso-Seq) is a type of RNA sequencing (RNA-Seq) method that uses Single Molecular, Real-Time (SMRT ®) long-read technology to sequence contiguous, full-length …
ISOdb: A Comprehensive Database of Full-Length Isoforms …
To explore the distribution of Iso-Seq reads for a given gene, ISOdb provides a genome browser to query and visualise the full-length read coverage and the transcript isoforms.
Step-by-Step Guide to Iso-Seq Protocol: From Sample Preparation …
Iso-Seq protocol includes five main steps: the transformation from RNA to cDNA, the construction of cDNA to SMRTbell library, sequencing with Sequel system, generating consensus cyclic …
Full-Length Transcripts Sequencing (Iso-Seq) - CD Genomics
Oct 31, 2020 · CD Genomics utilizes PacBio SMRT technology for Full-Length Transcript Sequencing (Iso-Seq). This advanced method involves extracting high-quality RNA, …
WHAT IS THE ISO-SEQ METHOD? The PacBio Iso-Seq method is an end-to-end workflow for sequencing and analyzing full-length transcript isoforms. WHY IS FULL-LENGTH RNA …
Introduction to Iso-Seq: Unveiling the Full-Length Transcripts with ...
Uncover the secrets of full-length transcripts with PacBio's Iso-Seq! The third-generation sequencing tech offers high-accuracy, complete transcript data. Learn about its principle, …