The IRES (internal ribosome entry site) allows the researcher to co-express several genes under the control of the same promoter. Within the past decade, the IRES has been used to develop hundreds of genetically modified models, and is considered to be the "go-to" technology for transgene co-expression in rodent animal models. How does it work?

012704 B6(Cg)- Crh tm1(cre)Zjh /J CRH-ires-CRE knock-in mice have Cre recombinase expression directed to CRH positive neurons by the endogenous promoter/enhancer elements of the corticotropin releasing hormone locus ( Crh ).

Plasmid CAG-GFP-IRES-CRE from Dr. Fred Gage's lab contains the inserts eGFP and Cre and is published in J Neurosci. 2006 Jan 4. 26(1):3-11. This plasmid is available through Addgene.

012899 STOCK Agrp tm1(cre)Lowl /J AgRP-IRES-Cre knock-in mice have IRES-Cre inserted downstream of the stop codon of the agouti related neuropeptide locus. Cre recombinase expression is observed in agouti-related protein secreting neurons.

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

The new Foxg1-IRES-Cre mouse line is a unique and advanced tool for studying genes involved in the development of the telencephalon from early embryonic stages. The telencephalon specific Cre activity in the brain and early timing of robust Cre activation (E9.0) in the telencephalon are remarkable advantages compared to other Cre lines ...

For the Kl model, the "IRES-CreERT2" cassette will be inserted 60~100 bp downstream of the TGA stop codon. The insertion site of the Cre recombinase gene expression cassette in this strain is located on chromosome 9. Please avoid breeding with gene-edited mice targeting genes on the same chromosome as the Cre tool mouse when conducting mating.

Analysis of double heterozygote embryos clearly demonstrates that Cre recombinase is expressed in vivo from the EphA2 IRES-Cre allele, and that the conditional reporter locus is efficiently restored in EphA2-expressing cells as early as 7.5 dpc.

These mice may be useful to study neural GABAergic circuits throughout the mammalian brain. While Vip-IRES-Cre was designed to retain endogenous Vip expression, a 2019 publication indicates this allele has reduced Vip expression - see details below.

Oct 23, 2022 · Here we report in the mouse cerebellar cortex that PV-Cre activity is not restricted to inhibitory neurons. Imaging of Cre-activated reporters demonstrated recombination in excitatory granule cells.