Immuno-PCR: A high-sensitivity approach for biomarker analysis
1 day ago · In the detection of parasitic infection markers, iPCR technology also shows its value. For example, studies by Mardani-Kataki et al. have shown that the Kappa value coefficient …
Understanding the IPC in DNA Quantitation Assays - Promega …
Ideally, the IPC serves two functions in the DNA workflow: 1) a means to assess reliability of the multiplexed DNA quantification assay(s) (i.e., flag a potential false negative or erroneously low …
Is Immuno-PCR Better than ELISA Test for Detection of
Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. …
Immuno-PCR Assays - Creative Diagnostics
Mar 13, 2025 · The limit of detection (LOD) for IPCR is defined as the average Ct value of the negative controls (wells containing buffer or sample matrix only without any antigen) plus three …
Is Immuno-PCR Better than ELISA Test for Detection of ... - PubMed
Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. …
Development of the covalent antibody-DNA conjugates …
The average background value of Cq (PAA) for all conjugates during iPCR was 22.7–24.6. Fig 7 shows the results of testing the conjugates in the iPCR on a panel of samples with a known …
Immuno-PCR: high sensitivity detection of proteins by nucleic acid ...
Taking advantage of specific conjugates comprising an antibody and a DNA marker fragment, IPCR combines the versatility of enzyme-linked immunosorbent assays (ELISAs) with the …
Immuno-PCR assays for immunogenicity testing - PubMed
Sep 18, 2009 · We here report on the employment of the ultra-sensitive immuno-PCR (IPCR) method to facilitate immunogenicity testing using two established assay formats.
Immuno-PCR: a promising ultrasensitive diagnostic method to …
Immuno-PCR (iPCR) is a method that combines the advantages of both enzyme-linked immunosorbent assay and PCR and is a powerful method for detecting low quantities of …
Enhanced Detection of Host Response Antibodies to
iPCR demonstrated a strong correlation with a commercial ELISA for detection of host antibodies in human serum using the VlsE C6 peptide. As recommended by the CDC, the first step of two …
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