Oct 27, 2019 · This is a protocol for the dual visualization of microglia and infiltrating macrophages in mouse brain tissue. TMEM119 (which labels microglia selectively), when combined with IBA1 (which provides an exceptional visualization of their morphology), allows investigation of changes in density, distribu …

This video covers the step by step protocol for IBA1 immunohistochemistry to target and visualize microglia. Demonstration by Fiona Ramnaraign. Video made by...

In a first step, we analyzed the progression of microglia activation in the medial corpus callosum using the (i) quantification of anti-ionized calcium-binding adaptor protein-1 (IBA1) + cell numbers, (ii) densitometrical analyses of IBA1 staining intensities, and (iii) morphological changes of IBA1 + cells by determining the cell ramification ...

"Anti Iba1, Rabbit (for Immunocytochemistry)" is an excellent microglial marker antibody since it can stain even microglial processes. Here, we describes the protocol and points to note when performing microglial immunohistochemistry.

Iba1 is a reliable cytoplasmic microglial marker with a strong signal, labelling both cell bodies and processes (Fig. 3, Step 1, Supplementary Fig. 2a). Nevertheless, there is a major pitfall in using this marker, since Iba1 does not exclusively label microglia cells.

We immunostained with anti-Iba1 antibody as our primary interest, but also stained some sections in parallel with 4G8 antibody for anti-Aβ staining comparison. Iba1 immunoreactivity was best enhanced by boiling in the low pH EDTA solution for both free floating and slide mounted tissues.

Aug 15, 2017 · Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in microglia with different phenotypes, ranging from ramified to amoeboid, to foamy phagocytizing macrophages, in adjacent sections immunocytochemically double stained for two of the markers.

Oct 27, 2019 · IBA1 is a calcium binding protein that provides exceptional visualization of microglial morphology, including fine distal processes, as confirmed by electron microscopy 6. This tool has been instrumental in characterizing microglial transformation, formerly called "activation", in a vast array of animal disease models 7,8,9.

Mar 14, 2025 · Immunohistochemical (IHC) staining for IBA1 is widely used to visualize myeloid cells in tissue sections. The process begins with tissue fixation, typically using paraformaldehyde or formalin, to preserve structures while maintaining antigen integrity.

Often the cause of uneven stairing is poor perfusion. The cortex is stained well, and striatum bad because striatum separated by white matter. PFA does not penetrate there during additional...