The mTORC2 pathway enhances the expression of Irf4, which is crucial for metabolic reprogramming in M2 macrophages. IRF4 has been reported to play roles in alternative macrophage activation (Satoh et al., 2010) and in mTOR-mediated regulation of glycolysis in CD8 + T cells (Man et al., 2013; Yao et al., 2013). We hypothesized that IRF4 could be ...

Jun 18, 2021 · IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay.

Jun 11, 2006 · Here we report that the transcription factor IRF4 is required for the generation of plasma cells. Transgenic mice with conditional deletion of Irf4 in germinal center B cells lacked...

High-dimensional single-cell profiling of T cells from chemotherapy-naive individuals with non–small-cell lung cancer identified the transcription factor IRF4 as specifically expressed by a subset of intratumoral CD4 + effector Tregs with superior suppressive activity.

Nov 26, 2019 · Plasma cells can provide lifelong immunity by the continued production of high-affinity, antigen-specific antibody. Low et al. reveal that the transcription factor IRF4 acts within established plasma cells to prevent apoptosis and maintain …

Aug 1, 2009 · To define additional factors that might contribute to Th2 heterogeneity, we examined the PU.1 interacting protein IFN-regulatory factor (IRF)-4. When Th2 cells are separated based on levels of IL-10 secretion, IRF4 expression segregates into the subset of Th2 cells expressing high levels of IL-10.

Herein, we investigate the role of transcription factor interferon regulatory factor 4 (IRF4) in controlling B cell response during chronic transplant rejection. We generated the Irf4gfp reporter mice to determine IRF4 expression in B cell lineage.

Oct 1, 2021 · Gene expression profiling of IRF4-deficient B cells at discrete divisions revealed IRF4 was critical for inducing MYC target genes, oxidative phosphorylation, and glycolysis. Moreover, IRF4-deficient B cells maintained an inflammatory gene expression signature.

6 days ago · AMO1 shIRE1 Cl.1 EV or shIRE1 Cl.1 IRF4 Cl. 4 and shIRF4 Cl.1 EV or shIRF4 Cl.1 IRF4 Cl. 5, as well as shNTC +EV and shNTC IRF4 Cl. 3 cells were incubated in the absence or presence of Dox (0.1 μg/mL) for the indicated time, EtOH-fixed and stained with PI before they were analyzed by flow cytometry.

May 27, 2021 · IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay.