Aug 22, 2010 · M2 macrophages are characterized by their high expression of arginase-1 (Arg1), chitinase-like Ym1 (Chi3l3), found in inflammatory zone-1 (Fizz1, also called Retnla) mannose receptor (Mrc1...

We found that IL-4 induced expression of CD301, RELMα, Arg1, Ym1, Il10, Lipa, Cd36, Fabp4, Pparg and Ppargc1b was diminished when Rictor was deleted (Fig.2G; S2H). In contrast, expression of CD301 and RELMα was increased over control M2 levels when Raptor ΔMΦ macrophages were stimulated with IL-4 .

Oct 18, 2016 · IL-4 and CSF-1 promote glucose metabolism during M2, or M (IL-4), macrophage activation. Macrophage activation status is intrinsically linked to metabolic remodeling.

Sep 25, 2019 · As expected, Trim24 ablation significantly promoted the expression of M2 signature genes in TAMs, including Arg1, Ym1, Mrc1, and Fizz1, and the macrophage-recruiting chemokine Ccl24 (Fig. 7h–j).

Generally, TAMs mainly refer to M2 macrophages, which are characterized by high expression of arginase1 (Arg1), chitinase-like 3 (Ym1/Chil3), found in inflammatory zone 1 (Fizz1) and mannose receptor (MR) (109, 110). IRFs play a key role in macrophage maturation …

May 2, 2013 · STAT6-mediated signaling by IL-4 results in enhanced expression of IRF4. IRF4-deficient macrophages likewise stimulated show diminished expression of genes associated with the M2 phenotype, including Il1rn.

Oct 12, 2023 · Previous studies have demonstrated that transcription factors such as STAT3, STAT6, C/EBPβ and IRF4 can manipulate macrophage plasticity by upregulating M2-related genes, especially Arg1.

Here we show that IRF4 expression is regulated in primary macrophages and in ATMs of high-fat diet–induced obese mice. Irf4−/− macrophages produce higher levels of proinflammatory cytokines, including interleukin-1β and tumor necrosis factor-α, in response to fatty acids.

Apr 1, 2025 · Interferon regulatory factor 4 (IRF4) siRNA was applied to knockdown IRF4 expression. M2-specific markers were quantified using quantitative real-time PCR (qRT-PCR), enzyme-linked immunosorbent assay (ELISA) and flow cytometry.

May 15, 2019 · Studies have demonstrated the importance of a GM-CSF→IFN regulatory factor 4 (IRF4)→CCL17 pathway, first identified in monocytes/macrophages, for arthritic pain and disease development. In this study, we further investigated the involvement of this new pathway in shaping the inflammatory response using the zymosan-induced peritonitis (ZIP) model.