Dec 2, 2021 · In this paper we report on the development of a method to obtain human neurons with the GCaMP6s calcium indicator, based on a human iPSC line with the TetON–NGN2 transgene complex.

In this paper we report on the development of a method to obtain human neurons with the GCaMP6s calcium indicator, based on a human iPSC line with the TetON–NGN2 transgene complex.

Here, we introduce a human Na + -activated K + (K Na) channel variant (KCNT1 -Y796H) into mice and, using a multiplatform approach, find motor cortex hyperexcitability and early-onset seizures, phenotypes strikingly similar to those of human patients.

In this paper we report on the development of a method to obtain human neurons with the GCaMP6s calcium indicator, based on a human iPSC line with the TetON–NGN2 transgene complex.

2 days ago · For GCaMP virus infection of four-part ... Neuron 97, 806–822.e10 (2018 ... B. et al. Human iPSC-derived trigeminal neurons lack constitutive TLR3-dependent immunity that protects cortical ...

To identify targets for managing hyperexcitability, a pathological feature of amyotrophic lateral sclerosis (ALS), we design a multi-step screening funnel using patient-derived motor neurons.

5 days ago · The development of induced pluripotent stem cell (iPSC) technologies in the last two decades has led to unprecedented advances in human CNS disease modeling and drug discovery1,2. Human iPSC-derived neurons provide an excellent system to assess neuronal function in in vitro physiological and pathological conditions.

1 day ago · a Neuron projections obtained from tdTomato- and GCaMP-corresponding channels using a dual-color microendoscope. b Spatial correlation of tdTomato and GCaMP projections at different time points.

Sep 24, 2023 · Results: The retro serotype has been found to be the most efficient in driving the expression of the GCaMP6f and is compatible with multi-time point neuronal Ca 2+ imaging in our human iPSC-derived neural cultures. An AAV2/retro carrying GCaMP6f under the hSyn promoter (AAV2/retro-hSyn-GCaMP6f) is an efficient vector that we have identified.

2 days ago · Human-Induced Pluripotent Stem Cell (iPSC) Neurons (iN) Culturing and Maintenance. iPSCs were differentiated to neurons of cortical layers V and VI based on Shi et al., 2012 with minor modifications [27, 28].A schematic illustrating the experimental protocol is provided in Fig. S1.Briefly, iPSCs were seeded in 6-well plates coated with Matrigel, at a density of 3 × 10 5 cells/cm 2 in E8 ...