In this study, 433 iPSC lines were differentiated into motor neurons using an optimized 32-day protocol selected based on extensive discussions with the scientific community. 6 Neuronal cell marker staining and gene expression patterns were analyzed in motor neuron cultures.

Apr 19, 2023 · Answer ALS has generated over 1,000 iPSC lines from control and amyotrophic lateral sclerosis (ALS) patients along with clinical and whole-genome sequencing data. The current report summarizes cell marker and gene expression in motor neuron cultures derived from 92 healthy control and 341 ALS participants using a 32-day differentiation protocol.

Aug 20, 2018 · Nature Medicine - iPSC-derived motor neurons from over 30 heterogeneous sporadic ALS cases exhibit pathologies correlated with clinical disease progression, are more similar to FUS/TDP-43...

Sep 26, 2023 · In iPSC-derived lower motor neurons, but not other derived cell types, we detect the downregulation of genes affected by TDP-43-dependent splicing. This reduction takes place exclusively within genotypes known to involve TDP-43 pathology.

Human iPSC-derived spinal alpha motor neurons (hiPSC-MN) have been generated by numerous protocols and validated by way of appropriate morphological characteristics, protein markers, transcription profiles, and electrophysiological measures.

Induced pluripotent stem cells (iPSCs) can generate a theoretically limitless number of MNs from a specific patient, making them powerful tools for studying MN diseases. However, to reach their potential, iPSCs need to be directed to efficiently differentiate into functional MNs.

We show successful differentiation in two unrelated iPSC lines, by quantifying neural-specific marker expression, and by evaluating cell functionality at different maturation stages by calcium imaging and patch clamping.

In this study, we characterized the electrophysiological properties of commercially available iPSC-derived motor neurons. Immunofluorescence confirmed the expression of motor neuron-specific biomarkers, indicating successful differentiation and maturation.

Dec 1, 2020 · Here we describe a combination of motor neuron (MN) derivation and single-cell RNA sequencing approaches to generate and characterize specific MN subtypes obtained from human iPSCs. Our studies provide evidence for rapid and robust generation of MN progenitor cells that can give rise to a heterogenous population of MNs.

In this article, 433 iPSCs from control and ALS patients were differentiated into motor neurons and profiled by immunocytochemistry and RNA-seq. Results reveal sex and cell composition to be among the strongest drivers of gene expression variation, with a higher percentage of Islet1+ cells observed in male ALS patient-derived cultures.