IL-1β treatment enhanced the ability of MSC to recruit monocytes and granulocytes in vitro. Blockade of NF-κβ transcription factor activation with IκB kinase beta (IKKβ) shRNA impaired MSC migration, adhesion and leucocyte recruitment, induced by IL-1β demonstrating that NF-κB pathway is an important downstream regulator of these responses.

Jan 15, 2017 · In this study, AF cells were exposed to IL-1β, which was applied to mimic intervertebral disc degeneration (IDD) in vitro. Indirect co-culture with BMSCs in a transwell co-culture system reduced the activity of nuclear factor-κB-p65 (NF-κB-p65) through the restoration of its inhibitor IκBa.

Exosomes derived from mesenchymal stem cells (MSCs) have shown promising therapeutic potential for various diseases. In this study, we aimed to investigate the action and mechanism …

Oct 6, 2022 · Bone marrow DCs in patients with low-risk myelodysplastic syndrome have high IL-1β and TLR1/2 expression. Hematopoietic stem/progenitor cells (HSPCs) reside in localized microenvironments, or niches, in the bone marrow that provide key signals regulating their activity.

Apr 29, 2021 · Here, we evaluated modulatory effects of curcumin-primed human (h)BMSC-derived EVs (Cur-EVs) on IL-1β stimulated human osteoarthritic chondrocytes (OA-CH). CellTiter-Blue Viability- (CTB), Caspase 3/7-, and live/dead assays were used to determine range of cytotoxic curcumin concentrations for hBMSC and OA-CH.

Dec 14, 2020 · Here, we investigate putative protective effects of hBMSC-derived EVs as a cell-free approach, on IL-1β-stimulated chondrocytes obtained from OA-patients. Methods: EVs were harvested from the cell culture supernatant of hBMSCs by a …

Mechanis-tically, we demonstrated that bMSC-derived exosomes contributed to the enhanced immunomodu-latory properties of bMSCs both in vitro and in vivo. Importantly, we found that miR-146a, a well-known anti-inflammatory microRNA, was strongly upregulated by IL-1b stimulation and selectively packaged into exosomes.

Oct 1, 2023 · In this study, we aimed to investigate the function and mechanism of exosomes derived from MSCs that were pretreated with IL-1β (IB-exos) in the context of septic ER …

Histological analyses indicated that tissues formed by BM-MSC exposed to low doses of IL-1β (10 and 50 pg/mL) were more intensely stained for cartilage specific matrix than tissues formed in...

Oct 6, 2022 · TLR1/2 agonist sensing by BMDCs results in increased production of the inflammatory cytokine IL-1β in the BM perivascular niche. Increased IL-1β levels are sensed by HSCs, leading to their pool expansion, biased myeloid differentiation, and …