Oct 21, 2014 · Below we provide a few methods to try and reduce background with mouse antibody on mouse tissue staining. Mouse on Mouse Polymer IHC Kit (ab269452) is a polymer-based system that provides increased sensitivity and detection simplicity and saves time.

Immunohistochemistry (IHC) is a popular technique used in mouse tissues to visualize the involvement of certain proteins within that tissue and how they respond to experimental conditions. One of the drawbacks of this, is that many antibodies (especially monoclonal antibodies) are produced in mouse.

1 day ago · Enabling translational research with anti-mouse tumor immunology antibodies for IHC Mouse models are valuable pre-clinical research tools. First, they are genetically and biologically similar to humans. Secondly, they mimic human disease states without the tissue sample limitations of human models.

Immunohistochemistry (IHC) using mouse retinal cryosections is widely used to study the expression and intracellular localization of proteins in mouse retinas. Conventionally, the preparation of retinal cryosections from mice involves tissue fixation, cryoprotection, the removal of the cornea and lens, embedding and sectioning.

Immunohistochemistry on mouse tissue utilizing mouse monoclonal antibodies presents a challenge. Secondary antibodies directed against the mouse monoclonal primary antibody of interest will also detect endogenous mouse immunoglobulin in the tissue. ...

Jan 23, 2024 · This protocol uses Fab Fragment Goat Anti-Mouse IgG (H+L) to avoid unspecific binding of secondary antibody to Fc receptors presented in mouse tissue, therefore eliminating the need to perform extra blocking step to block endogenous Fc receptors.

My Antibody Suggestions These are the IHC approved antibodies that I have optimized for staining in the Oliver Lab. Some of them are ideal and others may need further optimization. I have indicated the quality of the current conditions as either poor, fair, excellent.

Mar 25, 2024 · The Immunohistochemistry (IHC) Staining for Mouse Brain Sections protocol details the blocking, primary, and secondary antibody staining of 50-100 micron mouse brain tissue slices fixed in 4% PFA.

Jan 1, 2019 · In this study, we demonstrate multiplex IHC of mouse immune cell markers with an improved Ag retrieval protocol that works efficiently for most Abs. We used specific markers to detect populations of B cells, subtypes of T cells, NK cells, macrophages, dendritic cells (DCs), granulocytes, and total myeloid cells.

Mouse Phenotyping Services at the University of California, San Diego offers guidelines and some services, in order to proceed with a comprehensive investigation of genetically altered mice.