The molecular and cellular features of 2-cell-like cells: a …
Aug 26, 2020 · Here, we aim to provide an up-to-date list of 2CLC characteristics, including their transcriptional, chromatin and metabolic features. 2CLCs emerge spontaneously in ESCs, …
Pursuing totipotency: authentic totipotent stem cells in culture
The inner cell mass (ICM) cells in the blastocyst are pluripotent. The blastocyst has three distinct cell lineages: EPI-epiblast; PrE-primitive endoderm; TE-trophectoderm. EPI gives rise to all …
学术前沿：我国研究团队揭示2细胞样细胞 (2CLC)全能性维持新机制
Jun 6, 2022 · 大约 1% 的这些细胞，称为 2 细胞样细胞 (2CLC)，类似于 2 细胞阶段的卵裂球。 这些 2CLC 保留全能状态以产生胚胎和胚胎外组织，非常适合研究合子基因激活 (ZGA) 的分子 …
Hyperosmotic stress induces 2‐cell‐like cells through
Jul 11, 2023 · We show that hyperosmotic stress induces 2CLC and that this induction can occur even after a recovery time from hyperosmotic stress, suggesting a memory response. …
Chemical-induced chromatin remodeling reprograms mouse ESCs …
Mar 3, 2022 · Our approach of remodeling the pericentromeric heterochromatin and re-establishing the totipotency-specific broad H3K4me3 domains promotes the pluri-to …
On transposons and totipotency - PMC
Remarkably, one of the main features of these ‘2-cell-like cells’ (2CLC) is that they robustly transcribe elements from an LTR family of endogenous retroviruses, specifically MERVL …
Epigenetic regulation of mouse preimplantation embryo development
Oct 1, 2020 · 2CLC are a rare two-cell-embryo-like cell population in cultured mESCs, which can serve as an in vitro model for studying preimplantation development. Factors, such as …
NELFA and BCL2 induce the 2C‐like state in mouse embryonic …
Aug 17, 2023 · We found that Bcl2 OE ESCs contributed to both the ICM and the TE (Figure 6A,B). These observations imply that ESCs that overexpress Bcl2 exhibit expanded cell fate …
A cell in hand is worth two in the embryo: recent advances in 2-cell ...
Oct 1, 2020 · This paper studies the 2CLC reprogramming process to reveal cell trajectories identifying an intermediate state represented by the Zscan4+ population and uncovers new …
Jul 11, 2023 · Mouse embryonic stem cells (ESC) derive from the inner cell mass (ICM) of the blastocyst. Like the ICM, the majority of ESCs in cul-ture are pluripotent, which refers to the …