Jul 1, 2006 · The BrdU/HuC/D double labeled cells by far outnumber the BrdU/S100β double labeled cells, indicating that most of the cells in the proliferation zones are neurogenic and only few are gliogenic. We have also detected newborn cells expressing distinct neuronal phenotypes.

Sep 1, 2002 · This BrdU saturation-labeling shows – in conjunction with a Hu-assay demonstrating earliest neuronal differentiation – that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-labeled and double BrdU-/Hu-labeled cells in these aggregates.

Brains were analyzed at 7 days post injection (dpi) for BrdU (marker for proliferated cells), HuC (neuronal marker) and DAPI as a nuclear counterstain. (B) BrdU immunohistochemistry on the dorsal region of telencephalon from control morpholino-injected brain.

Nov 15, 2011 · To study brain regeneration mechanisms in adult zebrafish, we developed a traumatic lesion assay, analyzed cellular reactions to injury and show that adult zebrafish can efficiently regenerate brain lesions and lack permanent glial scarring.

Aug 11, 2021 · Combining our exercise protocol with the thymidine analog 5-bromo-2ʹ-deoxyuridine (BrdU), a marker of DNA synthesis, we observed a 3-fold increase in the number of BrdU + cells in the...

Here we show that the ubiquitin ligase Huwe1 operates upstream of the N-Myc-DLL3-Notch pathway to control neural stem cell activity and promote neurogenesis. Conditional inactivation of the Huwe1 gene in the mouse brain caused neonatal lethality associated with disorganization of the laminar patterning of the cortex.

Dec 2, 2015 · We have later characterized protein level expression of OCT4 by using antibody along with HuC/D, a neuronal marker and a proliferation marker, BrdU (Fig 5, S6 Fig and S4B Fig). We found that there are OCT4 + /HuC/D + neurons in the grey matter of uninjured cord ( …

Double labeling for BrdU and HuC was used to identify neurons that were born after BrdU injection. The average of neurons born after a specific time point was obtained by adding the number of double labeled neurons detected in each ganglion divided by the total number of ganglia analyzed.

Dec 17, 2024 · The percentage of BrdU-labeling in HuC/D + neurons was increased after the MSG treatment (Fig. 7 F). These results indicate that BrdU + proliferating cells differentiate into mature neurons in the AP of adult mice after MSG administration.

To investigate whether the increase in progenitor proliferation leads to neurogenesis, we performed bromodeoxyuridine (BrdU) pulse-chase analyses labeling the proliferating cells at earlier phases after TR-Aβ42 injection and determining their progeny (Figures 2D–2G).