HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.

The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively.

High-resolution structures revealed paired helical filament (PHF) and straight filament (SF) conformations of tau that were identical to those determined from AD cases. The PHFs and SFs are made of two C-shaped protofilaments, each containing a cross-β/β-helix motif.

Revvity’s line of HTRF KinEASE kits is based on our patented HTRF technology and can be used as a universal tool for assessing Serine/Threonine and Tyrosine kinase activity. HTRF KinEASE kits limit assay development time and are easily miniaturizable and flexible, meaning the assay can be performed under a wide range of kinase assay conditions,

A typical development for an HTRF ® KinEASE ™-TK assay consists of the following steps: 1. SEB titration 2. Enzyme titration 3. Kinetic study 4. Substrate titration 5. ATP titration 6. Biotin/streptavidin ratio optimization 7. Inhibitor IC50 determination Final concentrations of the assay components used for kinase assay optimization are ...

Revvity's human IFNγ assay is only intended for the quantitative measurement of IFNγ in supernatant using HTRF® technology. The assay is compatible with human samples, and is highly specific for IFNγ.

This kit is intended for the simple and rapid quantification of Native Histamine produced by cells in buffered solution, in cell culture supernatants or plasma and offers a fast alternative to ELISA.

PI 3-Kinase HTRF® Assay; 384 wells The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 1 plate.; Synonyms: Assay for PI 3-Kinase,HTRF Assay,PI 3-Kinase Assay at Sigma-Aldrich

This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2.

HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS).