Inflammation exerts multiple effects on the early hematopoietic compartment. Best studied is the role of proinflammatory cytokines in activating adult hematopoietic stem and progenitor cells to dynamically replenish myeloid lineage cells in a process known as emergency myelopoiesis.

Feb 22, 2023 · Here we report the establishment of a culture system that allows the long-term ex vivo expansion of human HSCs, achieved through the complete replacement of exogenous cytokines and albumin with...

Apr 1, 2025 · Here, we optimized a flow cytometry-based method for the simultaneous assessment of multiple intracellular cytokines in HSPCs. By selecting an optimal combination of cytokine restimulation reagents, protein transport inhibitors, and culture supplements, an optimized restimulation protocol for intracellular staining was developed.

Various cytokines have been used for in vitro maintenance and expansion of human HSPCs capable of repopulating immunodeficient mice, starting from the late 1990s [23,24]. The most widely used combination includes stem cell factor (SCF), thrombopoietin (THPO), Fms-like tyrosine kinase 3 ligand (FLT-3L) and interleukin-6 (IL-6) [ 25 ], which are ...

Sep 9, 2021 · We demonstrate heterogeneous cytokine- and cell-type–specific ERK signaling dynamics, illustrating their relevance in regulating hematopoietic stem and progenitor (HSPC) cell fates.

Apr 21, 2019 · HSPC mobilization involves a multifaceted and complex interaction of HSPCs and stromal and hematopoietic niche cells, as well as an array of cytokines, chemokines, and small molecules. Stem cell mobilization research has advanced immensely over the past decade.

Feb 28, 2023 · Wild-type microbiota promote HSPC development by controlling basal inflammatory cytokine expression in kidney niche, and chd8−/− commensals elicit elevated inflammatory cytokines that reduce HSPCs and enhance myeloid differentiation.

Oct 19, 2020 · Here, we review evidence of signals emerging from HSPCs through secreted autocrine or paracrine factors, including extracellular vesicles, and via direct contact within the niche. We also discuss...

Oct 7, 2019 · Here, using 3D culture of human HSPCs in a degradable zwitterionic hydrogel, we achieved substantial expansion of phenotypically primitive CD34 + cord blood and bone-marrow-derived HSPCs.

Apr 6, 2023 · The paucity of hematopoietic stem cells (HSCs) presents a challenge for both transplantation and the study of HSCs.1 Sakurai et al.2 now present a cytokine-free culture system for robust ex vivo expansion of functional human …