Mar 4, 2021 · The Act/HSPC signature contains CCCTC-binding factor (CTCF) binding sites mediating 351 chromatin interactions engaged in ST-HSCs, but not LT-HSCs, enclosing multiple stemness pathway genes active in LT-HSCs and repressed in ST-HSCs.

Jan 14, 2021 · Fine-tuning of chromatin accessibility primes HSPC transition from nascent to fetal states. Smarca5 interacts with nucleolin to promote chromatin remodeling and facilitate genomic binding of hematopoietic TFs in fetal HSPCs.

May 16, 2022 · Transposase-accessible chromatin sequencing (ATAC-seq) assays reveal that alterations in chromatin accessibility preferentially take place in HSCs with aging, which gradually resolve with...

Consistent with this interpretation, our single-cell ATAC-seq data confirm that the Act/HSPC signature based on chromatin accessibility shows an inverse relationship with the LT/HSPC signature, validating that the latter is progressively lost as the cells become more activated.

We developed an optimized protocol for use on primary blood cells, termed Fast-ATAC, which relies on a 1-step membrane permeabilization and transposition using the lysis reagent digitonin.

Here we applied single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow.

This is a paired-end ATAC-seq on human CD34+ HSPC investigating the effect of loss of CUX1 on chromatin accessibility in human HSPC. There are two consitions: gHPRT ctrl and gCUX1 CUX1-knockout. There are two replicates per condition.

Jan 14, 2021 · Our results unravel a new role of epigenetic regulation and reveal that Smarca5-mediated epigenetic programming is responsible for fetal HSPC development, which will provide new insights into the generation of functional HSPCs both in vivo and in vitro.

Dec 4, 2024 · Here we show that fetal liver hepatocytes provide protection to haematopoietic stem and progenitor cell (HSPC) genomes. Lineage tracing and depletion in mice demonstrated that delayed...

Aug 24, 2023 · Mechanistic studies incorporating CUT&RUN, ATAC-Seq and Hi-C identify ETV6 binding at inflammatory gene loci, including those within the TNF pathway in Etv6+/+ HSPCs, the mouse BM-progenitor derived HPC5 cell line, and G-CSF-mobilized human CD34+ cells.