Propidium iodide (PI) is used as a DNA stain by intercalating between the bases with little or no sequence preference. Once the dye binds to nucleic acid, its fluorescence is enhanced 20 to 30 fold. PI is excited at 488 nm and its maximum emission wavelength is 617 nm.

Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish …

Hoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA.

For double or triple fluorescence staining in immunofluorescence tests, the Hoechst 33342/PI staining is the last step after all fluorescence antibodies incubation;

Thermo Scientific Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460-490 nm.

The primary laser at 488nm to excite the propidium iodide and the secondary laser at ultraviolet (351.1 - 363.8 nm) to excite the Hoechst. Both signals are logarithmically amplified with PI fluorescence being detected above 600 nm and Hoechst fluorescence collected between 390 and …

The Double Stain Apoptosis Detection Kit (Hoechst 33342/PI) provides a rapid and convenient assay for apoptosis based upon fluorescent detection for the compacted state of the chromatin in apoptotic cells. This kit contains two ready-to-use dyes bound to DNA.

Cells emitting blue fluorescence were Hoechst positive while those with red fluorescence were PI positive. Although PI positive cells (dead cells) were rare in control group (a), their number increased significantly in C6-TK (b) and C6-sr39TK group (c) (C6-TK versus C6-sr39TK, P …

Viability Assay: oechst and Propidium Iodide (PI). Propidium Iodide is membrane impermeable and only enters the dead (membrane compromised) cells, where upon entry it binds o DNA in an intercalating manner. Hoechst 33342 is a DNA binding agent that is …

2. 2. Staining: Staining: Add Add 2.5 2.5 μL μL of of Hoechst Hoechst 33342 33342 staining staining solution solution and and 2.5 2.5 μL μL of of PI PI staining staining solution solution to to each each 1 1 mL mL of of cell cell suspension, suspension, mix mix gently gently and and incubate incubate in in an an ice ice bath bath protected protected from from light light for …