Jan 1, 2019 · His-tag of the BirA were designed at both N-terminal (His-BirA) and C-terminal (BirA-His), respectively. Immobilization of the His-BirA was performed, realized to 92.85% using by 10 mM H 2 O 2 without compromising catalytic activity.

MBP fusion protein with C-terminal MCS-TEV-AviTAG-HIS Vector expresses BirA (Biotin ligase) from E.coli.

2 μg/lane of RecombinantE. coliBirA His-tag (Catalog # 10464-BA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-38 kDa.

A facile and economical method was established for the oriented immobilization of biotin ligase (BirA) on Co 3+ -NTA sepharose through H 2 O 2 oxidation of Co 2+ and His-tag. His-tag of the BirA were designed at both N-terminal (His-BirA) and C-terminal (BirA-His), respectively.

BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor.

BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag). As a complementary approach to in vivo biotinylation of Avi-tag-bearing proteins, we developed a protocol for producing recombinant BirA ligase for in vitro biotinylation.

Plasmid BirA His-tag pLEMO from Dr. Alexander Gabibov's lab contains the insert Biotin ligase and is published in Bioconjug Chem. 2019 Apr 25. doi: 10.1021/acs.bioconjchem.9b00218. This plasmid is available through Addgene.

Apr 1, 2002 · A facile and economical method was established for the oriented immobilization of biotin ligase (BirA) on Co 3+-NTA sepharose through H 2 O 2 oxidation of Co 2+ and His-tag. His-tag of the BirA were designed at both N-terminal (His-BirA) and C …

Sep 1, 2018 · A facile and economical method was established for the oriented immobilization of biotin ligase (BirA) on Co³⁺-NTA sepharose through H2O2 oxidation of Co²⁺ and His-tag. His-tag of the BirA...

Enzymatic biotinylation with E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide, giving a homogeneous product with high yield. AviTag can conveniently be added genetically at the N-terminus, C-terminus, or …