The hemagglutination assay or haemagglutination assay (HA) and the hemagglutination inhibition assay (HI or HAI) were developed in 1941–42 by American virologist George Hirst as methods for quantifying the relative concentration of viruses, bacteria, or antibodies.

The sensitivity and specificity of microNT-ELISA assay were high (87% and 73%, respectively) and closely related to gold standard test results. Therefore, microNT-ELISA is recommended as an alternative or complementary test to conventional HI assay for …

The HI is a well-established gold standard method providing highly reproducible and reliable results. Other techniques such as ELISA may detect non-neutralizing antibodies, whereas the HI only detects antibodies which bind to the HA stem loop and thereby correlate with neutralization.

In this study, the three methods of choice: (i) the HI assay, (ii) an ELISA-based conventional MNT and (iii) a colorimetric MNT in terms of their ability to detect antibody responses in serum pairs collected from 43 healthy individuals before and 21 days after vaccination were compared.

Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard.

Hemagglutination inhibition test is widely used for the diagnosis of infection caused by orthomyxoviruses (influenza), paramyxoviruses (measles, mumps), mononucleosis, arboviruses-togaviruses (including rubella), flaviviruses, and bunyaviruses.

Hemagglutination-inhibition (HI) assay and a new affinity-purified enzyme-linked immunosorbent assay (ELISA) for detection of antibody to Mycoplasma gallisepticum (MG) compared for use as confirmatory tests for the National Poultry Improvement Plan program.

A single-serum dilution ELISA for egg drop syndrome (EDS) virus-specific antibodies was developed. In testing 425 chicken sera it was found to have a 93.6% sensitivity and 98.7% specificity relative to a hemagglutination inhibition (HI) test.

Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have ...

Yolks prepared by chloroform extraction and low-speed. centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the ELISA for NDV, IBV, and MG and by HI test for NDV.