To obtain a functional model of the human intestinal epithelium for studying toxin-induced disease mechanisms, differentiated enterocyte-like Caco-2 cells were co-cultured with goblet cell-like HT29-MTX cells.

HT29 MTX is a methotrexate-resistant cell line isolated from the original HT-29 human colon cancer cell line by adaptation to 10 µM Methotrexate. These cells present characteristics of mucous-secreting mature goblet cells.

Stepwise adaptation of exponentially growing HT29 cells to increasing concentrations of methotrexate (MTX) (up to 10 −5 mol) resulted in their transformation into mucus-secreting differentiated cells (Lesuffleur et al. 1990).

HT-29 is an adherent epithelial cell line isolated from a primary tumor obtained from a patient with colorectal adenocarcinoma. These cells express characteristics of mature intestinal cells and form a tight monolayer with an apical brush border exhibiting a similarity to enterocytes, making this line useful in gastrointestinal (GI) research.

In this study, the toxic effects (viability, inflammation, cellular monolayer integrity, modulation of cell type proportion and production of mucus) of four lipophilic phycotoxins (PTX2, YTX, AZA1 and OA) were evaluated in Caco-2/HT29-MTX co-cultured cells.

Sep 1, 2013 · Adhesion of Salmonella to HT29-MTX cell model was significantly higher, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface, compared to the non- and low-mucus producing Caco …

Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion.

Mar 6, 2017 · The obtained results suggested that the coculture of Caco-2/HT29-MTX and the triple coculture of Caco-2/HT29-MTX/Raji B were useful models to predict intestinal permeability and to classify the drugs in high or low permeability according to BCS.

Jul 3, 2020 · The aim of this study was to evaluate the suitability of the mucus-secreting HT29-MTX intestinal epithelial cell model to test adhesion and invasion of Salmonella strains and compare with data obtained with the more commonly used Caco-2 and HT-29 models.

Expression of sialyl-Tn antigen in breast cancer cells transfected with the human CMP-Neu5Ac:GalNAc alpha2,6-sialyltransferase (ST6GalNac I) cDNA. Glycoconj. J. 18:883-893 (2001)