Multi-parameter flow cytometry is a very important tool to phenotypically subdivide these hematopoietic stem and progenitor cell (HSPC) populations on the basis of cell surface marker expression and understand the diverse functions of different subsets of this heterogeneous group of …

Human HSCs are routinely analyzed by flow cytometry based on various extracellular and intracellular markers. The MC Antibody Cocktails simplify the flow cytometry analysis of cells separated by MACS Technology. All antibodies are optimally titrated to be simply added to an aliquot of a cell fraction before analysis.

Nov 22, 2022 · In murine models, HSCs are selected via flow cytometry through a combination of lineage-negative selection markers (lin−) and positive c-KIT and SCA-1 surface expression, resulting in a population of cells called lin− /SCA-1 + /c-KIT + (LSK) cells.

Jun 26, 2012 · In this article, we have reviewed some of the common methods for HSC purification by flow cytometry, discussed analysis of hematopoietic progenitors and peripheral blood samples of transplanted mice, and provided novel methods for working with limiting cell numbers when dealing with HSCs.

The HSC Flow Cytometry Core Facility is a full service, state of the art laboratory offering a complete spectrum of cytometric solutions. We provide access to spectral, conventional, and image based cytometers as well as a variety of cell sorters.

Learn how to phenotype LSK, LSK/SLAM, and ESLAM hematopoietic stem and progenitor cells from mouse bone marrow using flow cytometry.

Various studies have been conducted to identify hematopoietic stem cells (HSCs) using flow cytometry. The technique is primarily based on fluorochrome-conjugated antibodies against cell surface markers of HSCs and the physiological properties of HSCs such as high-efflux activity of certain fluoresce …

May 15, 2009 · During vertebrate embryogenesis, hematopoietic stem cells (HSCs) arise in the aorta-gonads-mesonephros (AGM) region. We report here that blood flow is a conserved regulator of HSC formation.

Dec 16, 2022 · Mitochondrial membrane potential (MMP) segregates functionally distinct subsets within highly purified hematopoietic stem cells (HSCs). Here, we detail a protocol for FACS isolation of MMP sub-fractions of phenotypically defined mouse and human HSCs.

Hemogenic endothelial cells undergo carefully orchestrated morphological adaptations during EHT. Moreover, expansion of the stem cell pool during embryogenesis requires HSC extravasation into the circulatory system and transit to the fetal liver, which is regulated by forces generated by blood flow.