Nov 26, 2020 · Key DAMPs for cell death to be perceived as immunogenic include calreticulin, HMGB1, ATP, ANXA1, and type I IFN. DAMP emission by cells undergoing ICD often relies on the activation of...

During an ICD event, HMGB1 translocates to the cytoplasm and eventually is released into the extracellular environment by either currently uncharacterized channel activation events or through passive release after a post-mortem loss of membrane integrity.

This system facilitates the identification of HMGB1 releasing agents in medium- to high-throughput screening assays. Keywords: Anthracyclines; Cancer; High-throughput screening; Immunogenic cell death (ICD); Retention using selective hooks (RUSH) assay.

High Mobility Group Box 1 (HMGB1) protein has been described as a consensus marker for immunogenic cell death (ICD) in cancer. To personalize treatments, there is a need for biomarkers to adapt dose prescription, concomitant chemotherapy, and follow-up …

Jan 19, 2022 · Glioblastoma (GBM) is the most lethal primary brain tumor with a dismal prognosis despite maximal therapy. The development of new therapies in this aggressive type of tumors remains highly...

These include defining the exact nature of the involvement of HMGB1 in ICD, either as a key mediator or as an impediment to the antitumor activities of other DAMPs released during this process.

Nov 11, 2020 · An important characteristic of ICD is the release of HMGB1 from the nucleus into the surroundings of dying cells (Fig. 2). HMGB1 mediates potent pro-inflammatory effects by binding to TLR4 on DCs to stimulate efficient processing and cross-presentation of tumor antigens from dying cells [[43, 50]]. In a tumor vaccination mouse model, using ...

Jul 13, 2020 · A tetrahydrobenzimidazole derivative TMQ0153 has a strong pro-oxidant effect against imatinib-sensitive and imatinib-resistant CML cells. TMQ0153 treatment significantly stimulates the release of HMGB1, leading to immunogenic cell death (ICD), which is a form of chemotherapy-induced tumor cell death . Yang et al. found that cytoplasmic HMGB1 ...

Kim et al. used this ELISA kit to measure the exosomal HMGB1 levels released from non-irradiated and gamma-irradiated melanoma cancer cells and demonstrated that exosomal HMGB1 stimulates immunogenic cell death (ICD). ICD is a form of cell death resulting in an activation of immune response.

Mar 1, 2024 · In this review, we outline the multifaceted functions of the HMGB1/RAGE axis, which encompasses tumor cell proliferation, apoptosis, autophagy, metastasis, and angiogenesis.