Mar 11, 2025 · Understanding how HL-60 cells respond to external signals provides insight into immune functions such as directed migration and coordinated cellular behavior. Cell Origin And Morphology. HL-60 cells were established in 1976 from the peripheral blood of a 36-year-old female with acute promyelocytic leukemia.

HL-60 is an immortal human leukemia cell line. It is widely used to study cancer biology and to explore the efficacy of anti-cancer drugs. Moreover, it can also be used to understand different cellular and molecular mechanisms involved in leukemic cell proliferation and differentiation.

HL-60 is a suspension cell line established from peripheral blood via leukapheresis from a patient with acute promyelocytic leukemia. These cells are promyelocytes but depending on culture conditions, HL-60 can differentiate into different lineages of mature myeloid cells; this makes it a useful model for immune response studies.

The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells.

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals.

In this chapter, we describe the maintenance and transient transfection of HL-60 cells and explain how to analyze their behavior with two standard chemotactic assays (micropipette and EZ-TAXIS). Finally, we demonstrate how to fix and stain the actin cytoskeleton of polarized cells for fluorescent microscopy imaging.

established from the peripheral blood of a 36-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described in the literature to be responsive to DMSO, phorbol ester TPA and other reagents (e.g. expression of TNF alpha) and to carry amplified MYC gene; present cells are app...

HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to …

The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established.

The cell line HL-60 was established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976. Recommended propagation method. Culture medium: 1. RPMI 1640 + 10% h.i. FBS 2. Iscove’s Modified Dulbecco’s Medium + 20% FBS . Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.