In this study, we examined whether microRNAs play a role in cyclosporine-induced nephrotoxicity. Treatment of mice with CsA resulted in nephrotoxicity that was associated with an early increase in...

Apr 7, 2023 · H 2 O 2 stimulation significantly increased the expression of α-SMA and suppressed E-cadherin expression levels, blockade of EZH2 inhibited upregulation of α-SMA and downregulation of...

Jun 22, 2009 · BMP6 ameliorated the TGF-β1-induced changes in HK-2 cells. The suppression of TGF-β1-mediated JNK and Smad2/3 signaling activation were implicated in these effects. Renal tubulointerstitial...

Jan 4, 2021 · Knockdown of HDAC5 ameliorated high glucose-induced epithelial–mesenchymal transition (EMT) of HK2 cells, indicated in the increased E-cadherin and decreased α-SMA, via the downregulation of...

Mechanical compression was associated with increased expression of Piezo1, fibronectin, and α-SMA in HK2 cells, while GsMTx4 could partially prevent these profibrotic alterations (Figure 5, I and J).

(A-C)The HK-2 cells under 30 mM glucose (B) or 30 mM glucose+cont siRNA (C) showed a loss of CK and E-cadherin and an increase of a-SMA and vimentin expression compared to that of the cells...

We investigated the effect of PTX3 on cultured human proximal tubular epithelial (HK-2) cells and a rat unilateral ureteral obstruction (UUO) model of renal fibrosis. Method... ...

Hexokinases (HKs) catalyse the first step in glucose metabolism, and HK2 constitutes the principal HK inducible isoform. We hypothesise that HK2 contributes to the synovial lining hypertrophy and plays a critical role in bone and cartilage damage.

Treatment with TGF-β1 reduced both the cell proliferation and the expression of E-cadherin, induced a morphological transformation, decreased the expression and activity of MMP-2, and increased the expression levels of α-SMA, fibronectin, and TIMP-2 in HK-2 cells.

We investigated gene and protein expression of HK-2 cells cultured in six medium formulations. TGF-β1 increased the expression of ACTA2, TGFB1, COL4A1, EGR2, VIM and CTGF genes while reducing PPARG in all medium formulations.