Sep 13, 2021 · HIV is known to have a significant genetic variability; therefore, achieving accurate quantification by real-time PCR may require primers and probes specific for each viral variant. An HIV database is available to download sequences for particular variants.

Further, we propose the establishment of a primer database for HIV-1 genomic sequencing that combines the primer walking strategies employed by individual laboratories into an internet accessible, global resource.

The APTIMA® HIV-1 RNA Qualitative Assay is an in vitro nucleic acid assay system for the detection of human immunodeficiency virus (HIV-1) in human plasma. It is intended for use as an aid in the diagnosis of HIV-1 infection, including acute or primary infection.

May 10, 2024 · HIV genotyping is used to assess HIV susceptibility to antiretroviral drugs. The Applied Biosystems HIV-1 Genotyping Kit with Integrase (AB kit, Thermo Fisher Scientific) detects resistance-associated mutations (RAMs) in HIV protease (PR), reverse transcriptase (RT), and …

The HIV-1 Detect™ HIV-1 primers amplify a conserved 100 base pair region of the HIV-1 genome encoding the Gag protein. The presence of RT-PCR products is measured by detection of the fluorescence emitting FAM- labeled probe. The unique features of the HIV-1 Detect™ Real Time PCR Kit include:

In this review, the various aspects of probe capture enrichment protocols used on HIV, and in some cases HCV, will be presented.

cDNA synthesis with primers specific for HIV could potentially yield greater overall coverage and sequencing depth. In an article by Henn et al. (16), for example, subtype B-specific primers were used to generate 4 overlapping amplicons (2 to 3 kb in length) that cover the entire genome and were then sequenced by 454 pyrosequencing.

The Applied BiosystemsTM HIV-1 Genotyping Kit with Integrase is designed to detect HIV genomic mutations in the Protease (PR) codons 6-99, Reverse transcriptase (RT) codons 1-251, and Integrase (IN) codons 1-288 regions of the pol gene in RNA isolated from human immunodeficiency virus type 1 (HIV-1).

TRUPCR® HIV Viral Load Kit is an in vitro nucleic acid amplification assay for the detection and quantification of Human Immunodeficiency type-1 (HIV-1) viral RNA in human serum or plasma (EDTA) from HIV infected individual specimens using Real Time PCR System.

Early detection allows for timely initiation of antiretroviral therapy, which can slow down the progression of HIV to AIDS. Available Pack size: Rxn: 25rxn, 50rxn, 100rxn. The kit includes an RT-PCR enzyme mix designed for RT-PCR technology, utilizing synthetic DNA primers & fluorescence-labeled probes.