The present study aimed to characterize the cell phenotypes of a panel of human breast carcinoma cell lines by measuring the expression levels of the markers classically used in clinic: ER, PR, HER2 and KI67, using IHC and immunocytochemistry (ICC).

Fluorescent single-cell analysis of SKBR3 breast cancer cells dual-labeled for EGFR and HER2 revealed a heterogeneous expression for receptors within both the cell population as well as within individual cells.

Jul 17, 2015 · Here, we describe a stoichiometric study that visualized HER2 at the molecular level in intact SKBR3 breast cancer cells, shedding light on its intra- and intercellular distribution patterns.

SKBR3, BT474, MDA-MB-231, and SKOV3 cells were evaluated for HER2 surface expression by direct staining of 1×10 6 cells in 100 µl of PBS (Lonza) with PE-conjugated anti-HER2/neu for 30 minutes at 4°C. Cells were then washed and analyzed for mean fluorescence intensity (MFI) by …

Dec 5, 2018 · Our findings show that lapatinib-bound HER2, complexed with HER3 via a non-canonical kinase dimer structure, induces higher order oligomers. We hypothesized that nucleation of receptors creates signaling platforms that explain the counterintuitive, increase in cell proliferation upon ligand binding, in the presence of the HER2-inhibitor lapatinib.

Results We found that HER2 expression was upregulated on chemoresistant cell lines, especially cisplatin-resistance (SBC-3/CDDP). In vitro, the rate of cell death increased with the amount of...

5 days ago · Generation of a reference standard. To create a set of reference samples to assess HER2 IHC scoring accuracy, samples were selected from a real-world cohort (N = 3750) of archival formalin-fixed, paraffin-embedded breast cancer tissue, acquired from biopsies, excisions, or resections.The cohort was made up of consecutive samples from patients with breast cancer tested between December 2018 and ...

Mar 16, 2017 · This work includes the IHC HER2+ clinical reference 3+ cell line SKBr3. We demonstrate that breast cancer cell lines and primary cells share many similarities regarding the phenotypic alterations (cell adhesion and temporal patterns) in response to HER family pathway agonists and antagonists when measured by CELx.

Nov 18, 2021 · Through genomic profiling of 733 HER2-amplified breast cancers, we identify enrichment of somatic alterations that promote MEK/ERK signaling in metastatic tumors with shortened progression-free...

Methods: Breast carcinoma cell lines SK-BR-3 and MCF-7 were characterized quantitatively by IHC with chicken anti-HER2 IgY antibody and by FISH with biotinylated bacterial artificial chromosome DNA probes; both assays used quantum dots as detectors.