Dec 5, 2019 · We found that ZEB1 is a critical EMT factor for mutant β-catenin-mediated loss of E-cadherin and Claudin-7 in HCT116-P and HCT116-MT cells.

In this study, we showed that TNFα induces EMT in human HCT116 cells and thereby promotes colorectal cancer (CRC) invasion and metastasis. TNFα-induced EMT was characterized by acquiring mesenchymal spindle-like morphology and increasing the expression of N-cadherin and fibronectin with a concomitant decrease of E-cadherin and Zona occludin-1 ...

Feb 15, 2016 · Here, we show that silencing or removing H2A.X, a histone H2A variant involved in cellular DNA repair and robust growth, induces mesenchymal-like characteristics including activation of EMT...

Feb 16, 2015 · In HCT116 human colon cancer cells treated with Dox (50 nmol/l), EMT was induced, and transforming growth factor (TGF)β signaling and multi-drug resistant plasma membrane glycoprotein levels were significantly increased.

Nov 8, 2019 · In this study, M1 macrophages (but not M2 macrophages) exhibited a potential to induce EMT in HCT116 and RKO cells through secretion of TNF-α and IL-1β, consistent with previous reports of...

The findings of the present study suggested that tetrandrine may inhibit EMT in IL-6-stimulated HCT116 cells; therefore, it may represent a potential drug for CRC.

Aug 21, 2021 · Tetrandrine significantly downregulated the expression and enzyme activity of MMP-2 in IL-6-stimulated HCT116 cells. In addition, tetrandrine restored E-cadherin gene promoter activity.

Apr 14, 2021 · Furthermore, Id4 overexpression upregulated the expression of proteins associated with cell proliferation, inhibited the PI3K/AKT pathway, and suppressed epithelial-mesenchymal transition (EMT) of HCT116 cells.

To more precisely elucidate EMT status in HCT116-P and HCT116-MT cells, we measured mRNA expression of SNAI1, SNAI2, ZEB1, TWIST1, VIM, and CDH2 (N-cadherin coding gene) under conditions of increasing cell density.

Mar 6, 2025 · In contrast, knockdown of MSLN or treatment with the WNT5A inhibitor Box5 in HCT116 cells could reverse the enhancing effects of CAFs-CTHRC1 on the migration, invasion, and EMT activity of HCT116 cells (Fig. 7 F, H, J).