The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are available, depending on the substrate used.

This week's focus on recombinant DNA technology features a protocol for the rapid separation of plasmid DNA, a baculovirus expression system and customized RNA-oligos.

The beta-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and computational analyses (including bioinformatics), its application in the study of plant gene expression is now an inte …

In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants. The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS).

What is virus associated DNA, and why do I have to order it? Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.

In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants. The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene …more

Gene fusions can be defined as DNA constructions (performed in vitro or in vivo) that result in the coding sequences from one gene (reporter) being transcribed and/or translated under the direction of the controlling sequences of another gene (controller). Most frequently, the reporter encodes an enzyme.

We have directed the evolution of a GUS variant that is significantly more resistant to both glutaraldehyde and formaldehyde than the wild-type enzyme. A variant with eight amino acid changes was...

Feb 27, 1995 · The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA sequences. Furthermore, the presence of a terminator sequence upstream from gus in pGUS2 should facilitate analysis and quantitation of expression from weak promoters.

In this chapter, I review several useful features of the GUS gene that make it a superior reporter gene for plant studies, and describe detailed protocols for the assay of beta-glucuronidase in transgenic plants. INTRODUCTION TO THE GUS SYSTEM The beta-glucuronidase of E. coli was first described in any