Engineered CRISPR systems contain two components: a guide RNA (gRNA or sgRNA) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic RNA …

A system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate cloning …

Invitrogen TrueGuide Synthetic gRNAs are ready-to-transfect CRISPR guide RNAs designed by a proprietary algorithm for high-efficiency editing. These predesigned, guaranteed single guide …

As shown in Figure 1, the CRISPR-Cas system relies on two main components: a guide RNA (gRNA) and CRISPR-associated (Cas) nuclease. The guide RNA is a specific RNA sequence …

Prime editing is a “search and replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing …

Feb 24, 2025 · We provide a guide to the design of the dsDNA template coding for gRNA elements for CRISPR–Cas9 base and prime editing, along with step-by-step instructions for …

CRISPR: Guide to gRNA design. Learn the steps involved in CRISPR ranging from designing your gRNA and repair templates to approaches to verify your genome edit.

Aug 17, 2015 · Here we report a simple, inexpensive, one-step method that allows for the rapid and efficient cloning of gRNA pairs into expression vectors. We show that this method can be …

Guide RNA (gRNA) or single guide RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9 -endonuclease or other Cas-proteins [1] that cut the double-stranded DNA …

Our guide RNAs are designed using a validated algorithm to achieve functional gene knockout with high specificity. By assessing phenotypes for thousands of designs, then validating our …