Oct 3, 2023 · We first investigated the binding specificity of the METTL3–METTL14 complex to a 27-nt single-stranded RNA (ssRNA) containing the GGACU modification sequence (ssRNA …

Nov 18, 2019 · A synthetic RNA oligomer (Trilink Biotechnologies) containing canonical and modified m6A bearing GGACU 5-mer was ligated to a carrier RNA. This was followed by in …

Oct 22, 2024 · Here, we introduce the m6A Transcriptome-wide Mapper (m6ATM), a novel Python-based computational pipeline that applies deep neural networks to predict m6A sites at …

To quantitatively analyze m6A modification, we divided the transcripts by their methylation motifs (GGACU, GACU, ACU, or GAC), which revealed that overall methylation decreased in the old …

Apr 5, 2023 · To date, transcriptome-wide m6A detection primarily relies on well-established methods using next-generation sequencing (NGS) platform. However, direct RNA sequencing …

FTO overexpression specifically removed m 6 A modification from GGACU and RRACU motifs in a concentration-dependent manner. These findings underline the dynamics of FTO in target …

Nov 24, 2023 · The characteristic sequence of m6A modification was GGACU and mainly concentrated in the coding sequence. According to the conjoint analysis with RNA-seq and …

Abnormal N6-methyladenosine (m6A) modification is closely associated with the occurrence, development, progression and prognosis of cancer, and aberrant m6A regulators have been …

In vitro methylation assays revealed that the METTL3/METTL14 complex catalyzed m 6 A deposition on GGACU motif-containing RNAs most efficiently, and photoactivatable …

Mar 22, 2022 · Furthermore, motif enrichment analysis showed that m 6 A peaks identified above shared a common sequence element “GGACU” (Figure 5A); thus, we intended to investigate …