* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases. Conclusion: The STR identification of this cell is correct.

The GL261 cell line cell line in Ubigene’s cell bank has low passages, high activity and good cell condition, with available STR Authentication reports.

1. Cell Name: GL261 2. Test Method: DNA was extracted using the genome extraction kit (Axygen), amplified using a 20- STR amplification protocol, the STR loci and gender gene Amelogenin were detected on the ABI 3730XL Genetic Analyzer. 3. Sample gene genotype analysis result: GenotypeanalysisresultsofSTRandAmelogeninlociincells STR Loci

gl261细胞是于1939年通过将3-甲基胆蒽颅内注射到c57bl/6小鼠中诱导产生的大脑胶质瘤细胞，在20世纪90年代中期从gl261肿瘤中建立体外生长细胞。 文献报道GL261细胞携带TP53和KRAS突变.

Jul 3, 2020 · GL261 murine glioma cells are widely used as a syngeneic animal model of glioma, however, it has become common practice to transfect these cells with luciferase for fluorescent tumor tracking.

• Routine authentication of tumor cell lines by STR profiling • Mycoplasma testing of tumor cells by PCR just prior to implantation • Routine health monitoring of sentinel animals (according to FELASA guide lines)

GL261）源自C57BL/6小鼠的神经胶质细胞。GL261细胞骨架染色突出呈现 肌动蛋白，在细胞质中有丰富的平行微丝。有报道称在1uM血管紧张素II存在下，它们会收缩。 细胞在软 琼脂中形成克隆，SV40大T抗原阳性。 GL261. 细胞系常用于胶质母细胞瘤（glioblastoma，GBM）的研究。

* 该细胞系与收录于 atcc, dsmz, jcrb 和 riken 数据库的细胞系 str 数据匹配。 结论：该细胞 str 鉴定正确。 细胞接收 1) 冻存细胞：如果是干冰运输的冻存细胞，收到后请立即转入液氮储存或短暂（24h)放至-80℃冰 箱保存，或直接进行细胞复苏。

源井生物提供的小鼠胶质细胞瘤 (GL261)由ATCC、ECACC和DSMZ等各大权威细胞库引进，细胞代次低、活性高、状态好，可提供STR鉴定报告，保证细胞身份准确。

Vaccine injection site matters: qualitative and quantitative defects in CD8 T cells primed as a function of proximity to the tumor in a murine glioma model. Profound impairment of adaptive immune responses by alkylating chemotherapy. A mass …