We applied histology, immunofluorescence, fluorescence‐activated cell sorting, flow cytometry, gene expression quantification, and 3D organoid cultures to whole tissue and single cells from the duodenum, jejunum, ileum, and colon of LGR5‐H2B‐GFP and wild‐type pigs.

Jan 1, 2012 · We have further extended the techniques of genetic engineering to rats, rabbits, and pigs, and have created corresponding GFP-transgenic animals. The GFP-positive organs of these animals provide valuable sensors in preclinical settings for …

Apr 2, 2013 · We have created a transgenic boar with green fluorescent protein (GFP) tagged 20S proteasomal core subunit α-type 1 (PSMA1-GFP), hypothesizing that the PSMA1-GFP fusion protein will be incorporated into functional sperm proteasomes.

Jun 27, 2019 · Fluorescence microscopy and genomic PCR analyses using pig mtDNA-specific and GFP primers showed that the PC-iPS cell derivatives could be detected in both mouse and pig pre-implantation blastocysts and post-implantation conceptuses.

Jun 10, 2023 · Here, we characterize GFP expression in the semilunar valves and great arteries of GFP-transgenic (GFP-Tg) pigs. Immunofluorescence was performed to visualize and quantify GFP expression and colocalization with nuclear staining.

To examine the chimera-forming potential of biPSCs in two different mammalian models, mice and pigs, we first introduced 10 GFP-labeled biPSCs into mouse embryos at 8–16 cell stage.

Aug 29, 2019 · Glomerulosclerosis was observed in transgenic mice and rabbits with ubiquitous reporter protein expression. Heart-specific GFP expression evoked dilated cardiomyopathy and altered cardiac function in transgenic mouse and zebrafish lines, respectively.

In this study, we investigated the feasibility of a simplified process of reconstituting the immune system of RAG2/IL2RG double-knockout SCID pigs by engrafting bone fragments from...

Dec 16, 2011 · Heterozygous transgenic pigs expressing GFP (GFP pigs) were compared to wildtype (wt pigs) littermates regarding health and development, social behaviour, exploratory behaviour and emotionality. Additionally heterozygous GFP sows (primiparous) were compared with wt sows regarding reproductory success and behavioural time budget.

We have further extended the techniques of genetic engineering to rats, rabbits, and pigs, and have created corresponding GFP-transgenic animals. The GFP-positive organs of these animals provide valuable sensors in preclinical settings for cell therapy and transplantation studies.