GFP to BFP Conversion: A Versatile Assay for the Quantification …
GFP to BFP conversion could be used to screen drug, mRNA or cDNA libraries for enhancers of HDR. Our data shows that a single 196T > C substitution using ssODN1 is sufficient to convert GFP to BFP .
A Guide to Fluorescent Protein FRET Pairs - PMC
Three main types of fluorophores have been used as FRET pairs in FRET biosensors: small organic dyes, fluorescent proteins (FPs), and quantum dots (QDs). Unlike dyes and QDs, FPs are genetically encoded and can be particularly useful in live cell FRET imaging.
A short guide on blue fluorescent proteins: limits and perspectives
Among FPs, the initially reported blue fluorescent protein (BFP) is closely related to green fluorescent protein (GFP). In the early 1960s, two scientists at the University of Washington tried to isolate a calcium ion–dependent bioluminescent protein (aequorin) from …
What is the difference between GFP and BFP? – SyronOptics
Green Fluorescent Protein (GFP) and Blue Fluorescent Protein (BFP) are both variants of the wild-type green fluorescent protein, originally isolated from the jellyfish Aequorea victoria. These proteins are widely used in molecular and cellular biology as markers for gene expression and protein localization.
fluoPEER-GFP>BFP - Addgene
Plasmid fluoPEER-GFP>BFP from Dr. Sabine Fuchs's lab contains the insert GFP-Y66-editing-site and is published in Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. This plasmid is available through Addgene.
GFP to BFP Conversion: A Versatile Assay for the ... - PubMed
Jul 12, 2016 · GFP to BFP Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing Mol Ther Nucleic Acids . 2016 Jul 12;5(7):e334. doi: 10.1038/mtna.2016.48.
Conversion of BFP to GFP enables detection of base
In this study, we utilized a blue fluorescent protein (BFP) that converts to green fluorescent protein (GFP) upon a C-to-T substitution as an assay to report directly on base...
Alignment of amino acid sequence of BFP and GFP. The two
Green fluorescent protein (GFP) is an unusually stable fluorescent protein that belongs to a family of related auto-fluorescent proteins (AFPs). These AFPs have been generated from jellyfish...
As an alternative, we propose a simple strategy for the simultaneous quantification of HDR and NHEJ by targeting the ubiquitous enhanced green fluo-rescent protein (EGFP) fluorescent reporter (Figure 1a, b).
五分钟教你花样玩转荧光蛋白 - 南模生物
Oct 15, 2020 · 1962年，维多利亚水母GFP首次被发现，在1992年被成功克隆，并在1994年首次作为荧光标记用于体内标记。 此后，水母衍生GFP被改造为蓝色（BFP）、青色（CFP）和黄色（YFP）突变体。 此外人们也陆续鉴定了来自其他物种的荧光蛋白，从而将其进一步扩展至橙色，红色和远红外光谱区（图2）。 图 2 荧光蛋白发展史 (A) 该领域阶段性成果时间表。 （B）FPs的系统发育树。 在培养皿上接种表达FPs的细菌，并用A型紫外线照射。 常用的荧 …