Oct 25, 2023 · Satellite glial cells (SGCs), enveloping primary sensory neurons' somas in the dorsal root ganglion (DRG), contribute to neuropathic pain upon nerve injury. Glial fibrillary acidic protein (GFAP) serves as an SGC activation marker, …

Injection of adeno-associated virus (AAV) vector conveying green fluorescent protein (GFP) transgene resulted in expression as soon as 1 day after injection into the DRG, including fibers in the spinal dorsal horn and columns.

Oct 14, 2014 · GFAP was up-regulated in rat DRG 14 days after STZ injection, as demonstrated by Western blot study. GFAP protein expression in STZ-treated rats was significantly increased in comparison with that in control rats.

Our results demonstrate an important discrepancy between species and experimental models that prevents the usage of GFAP as a universal marker for SGC reactivity. Keywords: satellite glial cells, GFAP, dorsal root ganglion, nerve injury, inflammation. 1. Introduction.

Aug 15, 2022 · Increase of glial fibrillary acidic protein (GFAP) immunoreactivity in DRG after SNI. (A) Representative tile-scan images and (B) high-resolution confocal images display DRG sections from Wistar rats at 14 d after SNI or sham operation.

Aug 28, 2018 · We found rises in GFAP + cells in the axotomized DRG indicating activation and potential turnover of SGCs. Several large caliber neurons had remarkable focal expansion of activated SGCs surrounding them indicating repopulation of SGCs around injured neurons ( …

Jul 1, 2020 · Glial fibrillary acidic protein (GFAP) was used as a marker of SGC activation. Unilateral C8VA resulted in mechanical allodynia, but not thermal hyperalgesia in bilateral paws.

Satellite glial cells (SGCs), enveloping primary sensory neurons’ somas in the dorsal root ganglion (DRG), contribute to neuropathic pain upon nerve injury. Glial fibrillary acidic protein (GFAP) serves as an SGC activation marker, though its DRG satellite cell specificity is debated.

The present study evaluated whether in vivo satellite glial cell (SGC) transduction in the DRG can be enhanced by the SGC-specific GFAP promoter and by using shH10 and shH19, which are engineered capsid variants with Müller glia-prone transduction.

Double immunofluorescence shows GFAP (red) and NeuroTrace (green). Note that GFAP expression after neural mobilization decreased when compared to CCI.