Oct 9, 2018 · Overall, RECK is a selective WNT7 receptor that mediates GPR124/FZD/LRP-dependent canonical Wnt/β-catenin signaling by stabilizing active cell surface WNT7, suggesting isoform-specific regulation of Wnt bioavailability.

Nov 9, 2023 · Through structural and functional analyses of Wnt7a, a crucial Wnt involved in central nervous system angiogenesis and blood-brain barrier maintenance, we have elucidated the principles of Wnt biogenesis and Wnt7-specific signaling.

Using a combination of biophysical approaches and ligand-binding assays in genetically engineered cells, we demonstrate that ligand selectivity is conferred by Reck, which mediates Wnt7-specific binding in a Frizzled-independent manner.

Oct 9, 2018 · Overall, RECK is a selective WNT7 receptor that mediates GPR124/FZD/LRP-dependent canonical Wnt/β-catenin signaling by stabilizing active cell surface WNT7, suggesting isoform-specific regulation of Wnt bioavailability.

GPR124 could potentially enhance WNT7 activity by aiding in the proper folding, biosynthesis or secretion of WNT7. Alternatively, GPR124 may act at the cell surface to augment the activity of functionally mature WNT7.

Aug 30, 2017 · Reck, a GPI-anchored membrane protein, and Gpr124, an orphan GPCR, have been implicated in Wnt7a/Wnt7b signaling in the CNS vasculature.

WNT7A and WNT7B control CNS angiogenesis and blood-brain barrier formation by activating endothe-lial Wnt/b-catenin signaling. The GPI-anchored pro-tein RECK and adhesion G protein-coupled receptor GPR124 critically regulate WNT7-specific signaling in …

Nov 9, 2024 · Through structural and functional analyses of Wnt7a, a crucial Wnt involved in central nervous system angiogenesis and blood-brain barrier maintenance, we have elucidated principles of Wnt biogenesis and Wnt7-specific signaling.

Reck-bound Wnt7 for Frizzled signaling relies on the interaction between Gpr124 and Dishevelled.Through polymerization, Dishevelled recruits Gpr124 and the associated Reck-bound Wnt7 into dynamic Wnt/Frizzled/Lrp5/6 signalosomes, resulting in increased local concentrations of Wnt7 available for Frizzled signaling.This work provides

Aug 17, 2018 · Structure-function analysis in zebrafish brain angiogenesis revealed that the intracellular domain is necessary, but not the transmembrane domain. This suggests that GPR124 acts as a signaling-deficient receptor protein in WNT7 signaling.