An example extracted-ion chromatogram from an LC-MS analysis. In an extracted-ion chromatogram (EIC or XIC), also called a reconstructed-ion chromatogram (RIC), one or more m/z values representing one or more analytes of interest are recovered ('extracted') from the entire data set for a chromatographic run. [3]

Aug 29, 2023 · (1) Explain the relationship between a total ion chromatogram (TIC), an extracted (or selected) ion chromatogram (XIC), and a tandem (MS/MS) mass spectrum. (2) Interpret GC-MS and LC-MS/MS data and predict/explain simple fragmentation patterns

The XIC intensity at a given point along the curve always appears to be higher that the fragment ion displayed in the ms/ms spectrum. Can you explain why this is the case? Please see attached example.

We present SWATHProphet software that identifies and quantifies peptide fragment ion traces in data-independent acquisition data, provides accurate probabilities to ensure results are correct, and automatically detects and removes contributions to quantitation originating from …

the presence of one or more precursor ions, collisionally fragment these ions, isolate, and detect the resulting fragment ions in sequential steps. The integration of extracted ion chromatograms (XIC) from the diagnostic fragment ions allows quantitation of the target.

Oct 30, 2013 · Label-free detection of specific oxidative modifications in any protein can be achieved with semi-targeted scanning routines, such as precursor ion or neutral loss scanning, which depend on diagnostic fragmentation patterns …

Jul 12, 2023 · MSFragger-DIA builds MS1 and MS/MS spectral indexes, which are then used to detect extracted ion chromatogram (XIC) features for all fragment and precursor peaks in a peptide-spectrum match...

Spectra and XIC can be generated by summing the data from pulses that correspond to isolation windows that encompassed the target m/z of interest. This simple processing reduces the intensity of fragment ions that come from other precursor m/z, …

extracted ion chromatogram (XIC) Chromatogram created by plotting the intensity of the signal observed at a chosen m/z value or set of values in a series of mass spectra recorded as a function of retention time

The fragments composed of one to four modified nucleotides (denoted as DZA) have been successfully recognised and transcribed to natural or modified RNA (denoted as RZA) by T7 RNA polymerase.